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Series GSE60840 Query DataSets for GSE60840
Status Public on Dec 01, 2014
Title miRCURY LNATM microRNA Array (7th Gen) profiling of monocyte-derived osteoclasts and macrophages.
Platform organisms Homo sapiens; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Cytomegalovirus; Lymphocryptovirus; Rhadinovirus; JC polyomavirus; Human immunodeficiency virus 1; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary To examine the miRNA changes that occur during osteoclast differentiation we used the miRCURY LNATM microRNA Array (7th Gen) to profile monocyte-derived osteoclasts and monocyte-derived macrophages. Of the 2046 probes present on the assay, we detected expression of ~400 (20%) miRNAs across all samples.
 
Overall design CD14+ monocytes were isolated from the peripheral blood of 4 normal, healthy volunteers and differentiated into osteoclasts or macrophages in vitro. Total RNA was isolated from these cells at 0, 24, 72, 144, and 288 hours of culture. RNA was profiled using the Qiagen miRCURY LNATM microRNA Hi-Power Labelling Kit and miRCURY LNATM microRNA Array (7th Gen).
 
Contributor(s) Fordham JB, Naqvi AR, Nares S
Citation(s) 27113904
Submission date Aug 27, 2014
Last update date Oct 07, 2019
Contact name Jezrom Bokcaerin Self-Fordham
E-mail(s) jez.fordham@gmail.com
Phone 7735247565
Organization name UIC
Department Periodontics
Lab 461
Street address 801 S Paulina
City Chicago
State/province IL
ZIP/Postal code 60612
Country USA
 
Platforms (1)
GPL19128 Exiqon miRCURY LNA microRNA array; 7th generation - hsa, mmu & rno (miRBase 18.0)
Samples (72)
GSM1489854 Osteoclast Donor 1 day 6 [1 Hy3TM]
GSM1489855 Osteoclast Donor 2 day 6 [2 Hy3TM]
GSM1489856 Osteoclast Donor 3 day 6 [3 Hy3TM]
Relations
BioProject PRJNA259652

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60840_ExpAnalysis1.xlsx.gz 197.8 Kb (ftp)(http) XLSX
GSE60840_ExpAnalysis2.xlsx.gz 167.6 Kb (ftp)(http) XLSX
GSE60840_ExpAnalysis3.xlsx.gz 197.6 Kb (ftp)(http) XLSX
GSE60840_ExpAnalysis4.xlsx.gz 167.4 Kb (ftp)(http) XLSX
GSE60840_ExpAnalysis5.xlsx.gz 88.8 Kb (ftp)(http) XLSX
GSE60840_ExpAnalysis6.xlsx.gz 88.7 Kb (ftp)(http) XLSX
GSE60840_ExpAnalysis7.xlsx.gz 89.7 Kb (ftp)(http) XLSX
GSE60840_ExpAnalysis8.xlsx.gz 90.7 Kb (ftp)(http) XLSX
GSE60840_RAW.tar 96.7 Mb (http)(custom) TAR (of TXT)
GSE60840_normalized_signal.txt.gz 333.2 Kb (ftp)(http) TXT
Processed data are available on Series record
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