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Series GSE60857 Query DataSets for GSE60857
Status Public on Dec 25, 2014
Title Coordinate Regulation of Enhancer Transcription at Super-Enhancers
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Other
Summary Using GRO-Seq, we find extensive regulation of enhancer RNAs (eRNA) within super-enhancers in response to lipopolysaccharide treatment in macrophages. Both activation and repression of gene expression are associated with super-enhancers and eRNA transcription dynamics. Treatment of macrophages with LPS targeted specific super-enhancers by attenuating their eRNA expression, leading to expression of key inflammatory genes. We propose that super-enhancers function as molecular rheostats integrating the binding profiles of key regulators to produce dynamic profiles of gene expression.
 
Overall design Nascent transcriptome (GRO-Seq) analysis over a time course (0, 20, 60, 180 min) of Lipopolisaccharide signaling in mouse bone marrow-derived macrophages.
 
Contributor(s) Hah N, Benner C, Yu R, Downes M, Evans RM
Citation(s) 25564661
Submission date Aug 27, 2014
Last update date May 15, 2019
Contact name Christopher Benner
E-mail(s) cbenner@ucsd.edu
Organization name University of California, San Diego (UCSD)
Department Medicine
Street address 9500 Gilman Dr. MC 0640
City La Jolla
State/province California
ZIP/Postal code 92093-0640
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (8)
GSM1490114 LPS-0min rep 1
GSM1490115 LPS-0min rep 2
GSM1490116 LPS-20min rep 1
Relations
BioProject PRJNA259674
SRA SRP045840

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60857_fpkm.txt.gz 1.5 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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