|
| Status |
Public on Sep 02, 2014 |
| Title |
miRNA profiling in the plasma of patients with in-stent restenosis (ISR) |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Studies of miRNA profiling in the plasma of patients between ISR and non-ISR
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| |
|
| Overall design |
Venous blood was collected in EDTA in the ward or the cardiac catheterization laboratory before the angiography procedure and heparin administration. Plasma was harvested by centrifugation and stored at -80°C until assayed. Identical volumes of plasma from the 6 patients with ISR and 4 patients with non-ISR were pooled to reach a final volume of 1500µL for each patient. Total RNA was extracted using miRVana isolation kit, dephosphorylated and labeled using miRNA Complete Labeling kit. Scanning was achieved with the illumina iScan System. The result were acquired with the Genome Studio (GenomeStudioV2009.1).
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| |
|
| Contributor(s) |
Li Y, He M |
| Citation(s) |
25427155 |
| |
| Submission date |
Sep 01, 2014 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Yue Li |
| E-mail(s) |
ly99ly@vip.163.com
|
| Phone |
86-451-85555673
|
| Organization name |
the First Affiliated Hospital of Harbin Medical University
|
| Department |
Cardiovascular Department
|
| Street address |
23 Youzheng Street
|
| City |
Harbin |
| State/province |
Heilongjiang |
| ZIP/Postal code |
150001 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL8179 |
Illumina Human v2 MicroRNA expression beadchip |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA259965 |
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