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Series GSE6097 Query DataSets for GSE6097
Status Public on Jan 03, 2007
Title Transcriptional analysis of a Vibrio choleare rpoH mutant vs wild-type after a heat-shock
Platform organism Vibrio cholerae O1 biovar El Tor str. N16961
Sample organism Vibrio cholerae
Experiment type Expression profiling by array
Summary Vibrio cholerae, the cause of cholera, can grow in a variety of environments outside of human hosts. During infection, the pathogen must adapt to significant environmental alterations, including the elevated temperature of the human gastrointestinal tract. σ32, an alternative sigma factor encoded by rpoH, activates transcription of genes involved in the heat-shock response in several bacterial species. We defined the V. cholerae RpoH regulon by comparing the whole genome transcription profiles of the wild-type and rpoH mutant strains after a temperature up-shift. Most of the V. cholerae genes expressed in an RpoH-dependent manner after heat-shock encode proteins that influence protein fate, such as proteases and chaperones, or are of unknown function.
Keywords: heat-shock response, rpoH
 
Overall design 3 independent experiments were performed, each in duplicate with dye swaps.
 
Contributor(s) Slamti L, Waldor MK
Citation(s) 17085549
Submission date Oct 20, 2006
Last update date Mar 16, 2012
Contact name Leyla Slamti
E-mail(s) leyla.slamti@tufts.edu
Organization name Tufts University/HHMI
Department Molecular biology and microbiology
Lab M. K. Waldor
Street address 136 Harrison Ave
City Boston
State/province MA
ZIP/Postal code 02111
Country USA
 
Platforms (1)
GPL3651 Vibrio cholerae Spotted oligonucleotides
Samples (6)
GSM105444 rpoH mt vs wt_heat shock (15-37ºC) Exp1.1
GSM105445 rpoH mt vs wt_heat shock (15-37ºC) Exp1.2
GSM105446 rpoH mt vs wt_heat shock (15-37ºC) Exp2.1
Relations
BioProject PRJNA97659

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6097_RAW.tar 2.9 Mb (http)(custom) TAR (of TXT)

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