NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE60973 Query DataSets for GSE60973
Status Public on Apr 08, 2015
Title MicroRNA-dependent transposable element transcriptional silencing in Drosophila somatic ovarian cells [RIP-Seq]
Organism Drosophila melanogaster
Experiment type Other
Summary RNAi-related silencing mechanisms concern as diverse biological processes as gene regulation, mostly via the miRNA pathway, and defense against molecular parasites, mainly controlled by the siRNA and the piRNA pathways. In Drosophila somatic ovarian cells, transposable elements (TEs) are repressed by chromatin changes induced by Piwi-interacting RNAs (piRNAs). We show here that a functional miRNA pathway is required for this piRNA-mediated TE transcriptional silencing to operate in this tissue. A general miRNA depletion, caused by tissue- and stage-specific knock-down of either drosha (involved in miRNA biogenesis) or AG01 and gawky (both responsible for miRNA activity) resulted in chromatin-mediated TE transcriptional desilencing and piRNA loss. For unknown reasons, the amount of piRNA produced by the traffic jam 3' UTR was apparently unaffected in miRNA-defective somatic ovarian cells. Although weaker, similar phenotypes could also be observed upon individual titration (by expression of the complementary miR-sponge) of at least three miRNAs, miR-14, miR-34 and miR-989. This work adds the maintenance of genome stability, via the piRNA-mediated TE repression, to the list of the already reported miRNA-controlled biological functions.
 
Overall design Analyses of RNA present in 3 different genetic backgounds with or without Drosha-IP: a control, a second one expressing the wild-type Drosha protein and a third one expressing the trans-dominant negative Drosha protein in the somatic cells.
 
Contributor(s) Mugat B, Akkouche A, Serrano V, Armenise C, Li B, Brun C, Fulga T, Van Vacto D, Pélisson A, Chambeyron S
Citation(s) 25993106
Submission date Sep 02, 2014
Last update date May 15, 2019
Contact name SEVERINE CHAMBEYRON
E-mail(s) severine.chambeyron@igh.cnrs.fr
Organization name CNRS
Department Institute of Human Genetics
Lab Non-coding RNA, epigenetics and genome stability
Street address 141, rue de la Cardonille
City MONTPELLIER
ZIP/Postal code 34396
Country France
 
Platforms (1)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
Samples (6)
GSM1494800 Input-tj-GAL4>Ø
GSM1494801 Input-tj-GAL4>WT-Drosha
GSM1494802 Input-tj-GAL4>TN-Drosha
This SubSeries is part of SuperSeries:
GSE60974 MicroRNA-dependent transposable element transcriptional silencing in Drosophila somatic ovarian cells
Relations
BioProject PRJNA260013
SRA SRP045962

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60973_RAW.tar 30.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.