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| Status |
Public on Sep 04, 2014 |
| Title |
Dormancy of green-cotyledon Col seeds matured at 20°C vs 15°C |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To determine genes involved in induction of dormancy in response to reduced maturation temperatures, RNA sequencing was performed on developing seeds matured at 20°C, where low dormancy is observed, and compared to seeds matured at 15°C where high dormancy is observed.
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| Overall design |
Col green cotyledon seeds matured at 20°C and 15°C were dissected from siliques between five to seven hours after dawn and frozen in liquid nitrogen. RNA was extracted as described previously (Penfield et al., 2005).
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| Contributor(s) |
MacGregor DR, Penfield S |
| Citation(s) |
25412428 |
| |
| Submission date |
Sep 03, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Dana Reid MacGregor |
| E-mail(s) |
dana.macgregor@rothamsted.ac.uk
|
| Organization name |
Rothamsted Research
|
| Department |
Biointeractions and Crop Protection
|
| Street address |
Rothamsted Research
|
| City |
Harpenden |
| State/province |
Hertfordshire |
| ZIP/Postal code |
AL5 2JQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA260171 |
| SRA |
SRP046088 |
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