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Status |
Public on Jan 27, 2015 |
Title |
The G1 arrest and maintenance mechanism between the long-term G1-arrested and the G1-arrested cells. |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Non mitotic tumor cells are resistant to conventional chemotherapeutic drugs. However, the mechanisms underlying this phenomenon remain unclear. Here, we found a population that is viable but remains in the G1 phase for an extended period of time (up to 48 h) by Long-term time-lapse observations in Fucci-HCT116 cells and then we conducted DNA microarray-based comparative analyses between the RR (the long-term G1-arrested cells) and R (the G1-arrested cells) fractions, to determine the molecular basis of the G1 arrest/maintenance mechanism. This study is related to GSE34940.
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Overall design |
The labeled cRNAs were hybridized on 4X44K v2 Agilent Whole Human Genome dual color Microarrays (G4845A) in two dye swap experiments, resulting in four individual microarrays.
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Contributor(s) |
Naito A, Kagawa Y, Okuzaki D |
Citation(s) |
25605732 |
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Submission date |
Sep 03, 2014 |
Last update date |
Jun 30, 2017 |
Contact name |
Daisuke Okuzaki |
E-mail(s) |
dokuzaki@biken.osaka-u.ac.jp
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Phone |
+81-6-6879-4935
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Organization name |
Osaka univ.
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Department |
Immunology Frontier Research Center
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Lab |
Human Immunology (Single Cell Genomics)
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Street address |
Yamadaoka 3-1
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City |
Suita |
State/province |
Osaka |
ZIP/Postal code |
565-0871 |
Country |
Japan |
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Platforms (1) |
GPL10332 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Feature Number version) |
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Samples (4)
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GSM1496386 |
R(cy3) vs RR(cy5) biological replicate 1 |
GSM1496387 |
RR(cy3) vs R(cy5) biological replicate 1 dyeswap |
GSM1496388 |
R(cy3) vs RR(cy5) biological replicate 2 |
GSM1496389 |
RR(cy3) vs R(cy5) biological replicate 2 dyeswap |
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Relations |
BioProject |
PRJNA260209 |