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| Status |
Public on Sep 05, 2014 |
| Title |
Expression data from primary rat aortic smooth muscle cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Electrochemical gradients of monovalent cations across the plasma membrane (high intracellular potassium, [K+]i vs low intracellular sodium, [Na+]i) are created by the Na+,K+-pump and determine a large variety of physiologically important processes. We hypothesized that transcriptomics changes triggered by hypoxia are at least partially caused by Na+i/K+i-mediated excitation-transcription coupling .
We used microarrays for identification and functional characterization of [Na+]i/]K+]i-sensitive transcriptome in RASMC
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| Overall design |
Quiescent cells were washed with K+-free DMEM and incubated for 6 hr at 37oC in a humidified atmosphere with 5% CO2/balance air in control DMEM, K+-free DMEM and DMEM containing 3 mM ouabain. Total RNA was extracted from cells grown in 6-well plates with TRIzol® reagent and purified with RNeasy® MinElute cleanup kit, following the manufacturers’ protocols. Microarray experiments were performed with GeneChip® Rat Gene 1.0 ST array.
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| Contributor(s) |
Orlov SN, Koltsova SV, Hamet P |
| Citation(s) |
25375852 |
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| Submission date |
Sep 04, 2014 |
| Last update date |
Dec 06, 2014 |
| Contact name |
Sergei Orlov |
| E-mail(s) |
SergeiNOrlov@yandex.ru
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| Organization name |
Moscow State Univeristy
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| Street address |
1/b.12 Leninskie Gory
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| City |
Moscow |
| ZIP/Postal code |
119991 |
| Country |
Russia |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA260301 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE61131_RAW.tar |
40.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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