|
| Status |
Public on Dec 05, 2008 |
| Title |
Gene expression in PAO1, clonal AES-1 and non-clonal isolates of Pseudomonas aeruginosa |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by array
|
| Summary |
Pseudomonas aeruginosa (P. aeruginosa) lung infection is a significant cause of mortality in patients with cystic fibrosis (CF). Most CF patients acquire unique P. aeruginosa strains from the environment; however clonal strains have been identified in CF communities in several countries. Two clonal strains infect 10% to 40% of patients in three CF clinics in mainland eastern Australia. The expression profiles of four planktonically-grown isolates of one Australian clonal strain (AES-1), and four non–clonal CF P. aeruginosa isolates were compared to each other and to the reference strain PAO1 using the Affymetrix P. aeruginosa PAO1 genome array, to gain insight into properties mediating the enhanced infectivity of AES-1. The isolates were subsequently grown as 3-day old biofilms and similarly extracted for RNA and compared as above. Data analysis was carried out using BIOCONDUCTOR software.
Keywords: Comparative strain hybridization
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|
| Overall design |
Four isolates of AES-1 strain were compared to four non-clonal isolates and to the reference strain PAO1, when grown both as planktonic and as a biofilm culture.
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| Contributor(s) |
Manos J, Arthur J, Rose B, Curtis M, Webb J, Kjelleberg S, Bye P, Harbour C |
| Citation(s) |
19222585 |
| Submission date |
Oct 24, 2006 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Jim Manos |
| E-mail(s) |
jim.manos@sydney.edu.au
|
| Phone |
+612 9351 8942
|
| Organization name |
University of Sydney
|
| Street address |
Camperdown
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2006 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
|
| Samples (18)
|
| GSM142284 |
Pseudomonas aeruginosa (non-clonal isolate 8) planktonic culture |
| GSM142285 |
Pseudomonas aeruginosa (non-clonal isolate 6) planktonic culture |
| GSM142286 |
Pseudomonas aeruginosa (non-clonal isolate 5) planktonic culture |
| GSM142288 |
Pseudomonas aeruginosa (non-clonal isolate 7) planktonic culture |
| GSM259816 |
Pseudomonas aeruginosa AES-1 (clonal isolate 1) planktonic culture |
| GSM259817 |
Pseudomonas aeruginosa AES-1 (clonal isolate 2) planktonic culture |
| GSM259818 |
Pseudomonas aeruginosa AES-1 (clonal isolate 3) planktonic culture |
| GSM259819 |
Pseudomonas aeruginosa AES-1 (clonal isolate 4) planktonic culture |
| GSM259820 |
Pseudomonas aeruginosa AES-1 (clonal isolate 1) biofilm culture |
| GSM259821 |
Pseudomonas aeruginosa AES-1 (clonal isolate 2) biofilm culture |
| GSM259822 |
Pseudomonas aeruginosa AES-1 (clonal isolate 3) biofilm culture |
| GSM259823 |
Pseudomonas aeruginosa AES-1 (clonal isolate 4) biofilm culture |
| GSM260365 |
Pseudomonas aeruginosa (non-clonal isolate 6) biofilm culture |
| GSM260366 |
Pseudomonas aeruginosa (non-clonal isolate 5) biofilm culture |
| GSM260368 |
Pseudomonas aeruginosa (non-clonal isolate 8) biofilm culture |
| GSM260370 |
Pseudomonas aeruginosa (non-clonal isolate 7) biofilm culture |
| GSM260371 |
Pseudomonas aeruginosa PAO1 planktonic culture |
| GSM260372 |
Pseudomonas aeruginosa PAO1 - biofilm culture |
|
| Relations |
| BioProject |
PRJNA97617 |
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