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| Status |
Public on Sep 15, 2014 |
| Title |
Characteristics of microRNA expression in mammary tissue of dairy and beef breed heifers |
| Organism |
Bos taurus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
MicroRNAs (miRNAs) are small noncoding RNAs that participate in regulation of gene expression. Their role during mammary gland development is still largely unknown. In the present study, we performed a microarray analysis to identify miRNAs associated with high mammogenic potential of bovine mammary gland. We identified 54 miRNAs differing significantly between mammary tissue of dairy (Holstein-Friesian, HF) and beef (Limousine, LM) post-pubertal heifers. Fifty two miRNAs had higher expression in the mammary tissue of LM heifers. Enrichment analyses for targeted genes revealed that the major differences between miRNA expression in the mammary gland of HF vs. LM were associated with regulation of signalling pathways crucial for mammary gland development, such as: TGF-beta, insulin, WNT and inflammatory pathways. Moreover, a number of genes potentially targeted by differentially expressed miRNAs was associated with mammary stem cells’ activity. These data indicate that in dairy cattle high developmental potential of the mammary gland, leading to high milk productivity, not only depends on central neuro-endocrine regulation but also on specific miRNA expression pattern.
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| Overall design |
miRNA profiling of Holstein Freisian (dairy breed) and Limousne heifers (beef breed) mammay glands. Two-condition experiment, LM (test) vs. HF (reference). Total RNA was isolated from quarters of 4 LM and 4 HFmammary glands.
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| Contributor(s) |
Wicik Z, Gajewska M, Majewska A, Osińska E, Motyl T |
| Citation(s) |
26060050 |
| |
| Submission date |
Sep 08, 2014 |
| Last update date |
Nov 17, 2015 |
| Contact name |
Zofia Wicik |
| E-mail(s) |
zofiawicik@gmail.com
|
| Organization name |
Mossakowski Medical Research Center
|
| Department |
Department of Human Epigenetics
|
| Street address |
Pawinskiego 5
|
| City |
Warsaw |
| State/province |
mazovia |
| ZIP/Postal code |
02-106 |
| Country |
Poland |
| |
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| Platforms (1) |
| GPL19170 |
Agilent-049625 Bos taurus_miRNA [Probe Name Version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA260542 |
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