|
| Status |
Public on Oct 27, 2006 |
| Title |
Amplification and overexpression of E2F3 in human bladder cancer. |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by array
|
| Summary |
We demonstrate that, in human bladder cancer, amplification of the E2F3 gene, located at 6p22, is associated with overexpression of its encoded mRNA transcripts and high levels of expression of E2F3 protein. Immunohistochemical analyses of E2F3 protein levels have established that around one-third (33/101) of primary transitional cell carcinomas of the bladder overexpress nuclear E2F3 protein, with the proportion of tumours containing overexpressed nuclear E2F3 increasing with tumour stage and grade. When considered together with the established role of E2F3 in cell cycle progression, these results suggest that the E2F3 gene represents a candidate bladder cancer oncogene that is activated by DNA amplification and overexpression.
Keywords: Tumour vs normal comparison, array CGH
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| |
|
| Overall design |
10 samples - 4 Bladder Cancer Cell lines and 2 Primary Bladder Tumours compared with Male Genomic DNA
|
| Web link |
http://www.crukdmf.icr.ac.uk/array/9/9.html
|
| |
|
| Contributor(s) |
Feber A, Clark J, Goodwin G, Dodson AR, Smith PH, Fletcher A, Edwards S, Flohr P, Falconer A, Roe T, Kovacs G, Dennis N, Fisher C, Wooster R, Huddart R, Foster CS, Cooper CS |
| Citation(s) |
14716298 |
| |
| Submission date |
Oct 25, 2006 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Ian Giddings |
| E-mail(s) |
ian.giddings@icr.ac.uk, daniel.brewer@icr.ac.uk
|
| Phone |
+44 20 8722 4293
|
| Fax |
+44 20 8722 4141
|
| URL |
http://www.crukdmf.icr.ac.uk
|
| Organization name |
Institute of Cancer Research
|
| Department |
Section of Molecular Carcinogenesis
|
| Lab |
CANCER RESEARCH UK DNA Microarray Facility
|
| Street address |
15 Cotswold Road
|
| City |
Sutton |
| State/province |
Surrey |
| ZIP/Postal code |
SM2 5NG |
| Country |
United Kingdom |
| |
|
| Platforms (3) |
| GPL3898 |
Institute of Cancer Research 5808-spot slide_version3 |
| GPL4482 |
3x19plt b 15 7 02 |
| GPL4483 |
5808-v4-1q |
|
| Samples (10)
|
| GSM142303 |
GS MGII NEW 5637 Rd v's sigma male Grn (1) 020802 |
| GSM142304 |
GS MGII NEW HT-1197 Rd v's sigma male Grn (1) merged 020802 |
| GSM142305 |
GS MGII HT-1376 Rd v's sigma male Grn (1) 020802 |
| GSM142306 |
GS MGII T110 Rd V's sgima male Grn merged. 160802 |
| GSM142307 |
GS MGII T63 Rd V's sgima male Grn merged 160802 |
| GSM142308 |
GS MGII NEW TCCSUP Rd v's sigma male Grn (1) 020802 |
| GSM142309 |
K 5637 Rd v's Male musc Grn (2)251101 |
| GSM142310 |
K HT-1376 Rd v's Male musc Grn 251101 |
| GSM142311 |
K TCCSUP Rd V's Male blood Grn 101101 |
| GSM142312 |
K TCCSUP Rd v's sigma male Grn (1) 240602 |
|
| Relations |
| BioProject |
PRJNA97619 |
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