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Series GSE61566 Query DataSets for GSE61566
Status Public on Oct 30, 2014
Title Isolation and Transcriptome Analyses of Human Erythroid Progenitors: BFU-E and CFU-E
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Third-party reanalysis
Summary Burst-forming unit-erythroid (BFU-E) and colony-forming unit-erythroid (CFU-E) cells are erythroid progenitors traditionally defined by colony assays. We developed a flow cytometry-based strategy for isolating human BFU-E and CFU-E cells based on the changes in expression of cell surface markers during in vitro erythroid cell culture. BFU-E and CFU-E are characterized by CD45+GPA-IL-3R-CD34+CD36-CD71low and CD45+GPA-IL-3R-CD34-CD36+CD71high phenotypes, respectively. Colony assays validated phenotypic assignment giving rise to BFU-E and CFU-E colonies, both at a purity ~90%. The BFU-E colony forming ability of CD45+GPA-IL-3R-CD34+CD36-CD71low cells required SCF and erythropoietin, while the CFU-E colony forming ability of CD45+GPA-IL-3R-CD34-CD36+CD71high cells required only erythropoietin. Bioinformatic analysis of the RNA-seq data revealed unique transcriptomes in each differentiation stage. The sorting strategy was validated in uncultured primary cells isolated from bone marrow and peripheral blood, indicating that marker expression is not an artifact of in vitro cell culture, but represents an in vivo characteristic of erythroid progenitor populations. The ability to isolate highly pure human BFU-E and CFU-E progenitors will enable detailed cellular and molecular characterization of these distinct progenitor populations and define their contribution to disordered erythropoiesis in inherited and acquired hematological disease. Our data provide important resource for future studies.
Overall design Transcription profiles of Human erythroid progenitors at distinct developmental stages were generated by deep sequencing, in triplicate, using IlluminaHiSeq 2000.
The complete dataset comprises 4 sample types: CD34, BFU, CFU, and Pro (reanalysis of GSM1304777-GSM1304779).
Contributor(s) Li J, Hale JP, Bhagia P, Xue F, Chen L, Jaffray J, Yan H, Lane J, Gallagher PG, Mohandas N, Liu J, An X
Citation(s) 25339359
Submission date Sep 19, 2014
Last update date May 15, 2019
Contact name John Hale
Organization name New York Blood Center
Lab Red Cell Physiology
Street address 310 East 67th Street
City New York
State/province NY
ZIP/Postal code 10065
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (9)
GSM1508256 hs_CD34_1
GSM1508257 hs_CD34_2
GSM1508258 hs_CD34_3
Reanalysis of GSM1304777
Reanalysis of GSM1304778
Reanalysis of GSM1304779
BioProject PRJNA261486
SRA SRP047323

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61566_cds.count_tracking.txt.gz 1.3 Mb (ftp)(http) TXT
GSE61566_cds.diff.gz 1.1 Mb (ftp)(http) DIFF
GSE61566_cds.fpkm_tracking.gz 1.9 Mb (ftp)(http) FPKM_TRACKING
GSE61566_cds_exp.diff.gz 3.3 Mb (ftp)(http) DIFF
GSE61566_gene_exp.diff.gz 2.2 Mb (ftp)(http) DIFF
GSE61566_genes.count_tracking.txt.gz 883.2 Kb (ftp)(http) TXT
GSE61566_genes.fpkm_tracking.gz 1.4 Mb (ftp)(http) FPKM_TRACKING
GSE61566_isoform_exp.diff.gz 4.0 Mb (ftp)(http) DIFF
GSE61566_isoforms.count_tracking.txt.gz 1.5 Mb (ftp)(http) TXT
GSE61566_isoforms.fpkm_tracking.gz 2.4 Mb (ftp)(http) FPKM_TRACKING
GSE61566_promoters.diff.gz 1.3 Mb (ftp)(http) DIFF
GSE61566_splicing.diff.gz 2.0 Mb (ftp)(http) DIFF
GSE61566_tss_group_exp.diff.gz 3.1 Mb (ftp)(http) DIFF
GSE61566_tss_groups.count_tracking.txt.gz 1.2 Mb (ftp)(http) TXT
GSE61566_tss_groups.fpkm_tracking.gz 1.7 Mb (ftp)(http) FPKM_TRACKING
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Raw data are available in SRA
Processed data are available on Series record

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