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| Status |
Public on Oct 05, 2015 |
| Title |
Bap1 loss results in EZH2 dependent transformation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Analysis of sorted granulocyte macrophage progenitors (GMPs) in control and Bap1-deficient bone marrow cells. Loss of Bap1 in the hematopoietic compartments results in an MDS-like disease. These data allow for the examination of the genetic underpinnings of Bap1 loss in disease.
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| Overall design |
Whole bone marrow cells from mice treated with polyI:polyC at three weeks of age were extracted from Mx1-Cre positive and Mx1-Cre negative Bap1f/f littermates. Bone marrow cells were red cell lysed, stained and sorted for GMP cells (Lineage-c-Kit+Sca1-CD34+Fc?). RNA was extracted for RNA-Seq studies to assess transcriptional changes with Bap1 loss in GMPs.
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| Contributor(s) |
LaFave L, Spitzer B, Socci N, Levine R |
| Citation(s) |
26437366 |
| |
| Submission date |
Sep 19, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Barbara Spitzer |
| E-mail(s) |
spitzerb@mskcc.org
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Lab |
Levine Lab
|
| Street address |
411 E 67th St
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA261525 |
| SRA |
SRP047331 |
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