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| Status |
Public on Nov 23, 2014 |
| Title |
Influence of osmopriming on rape seeds germination |
| Organism |
Brassica napus |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptomic study of the impact of osmopriming on rape seeds (Brassica napus L.; cv 'Libomir') during priming process and after germination. The assays were replicated twice on two independent priming and germination experiments.
Seeds were osmoprimed in PEG solution (-1.2 MPa osmotic potential) during 7 days, dried to initial moisture content and then germinated for 7 hours on water. The analysis during different phases of priming procedure (soaking and drying), after whole osmopriming process and germination were done.
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| Overall design |
10 samples, four condition experiment;
non dried primed seeds (Pnd) vs. dry unprimed seeds (UPd) (PEG soaking),
non dried primed seeds (Pnd) vs dry primed seeds (Pd) (drying after soaking),
dry primed seeds (Pd) vs. dry unprimed seeds (UPd) (full osmopriming process),
primed seeds imbibed on water (P7h) vs unprimed seeds imbibed on water (UP7h) (germination after osmopriming).
Biological replicates: 2 replicates for comparison PEG soaking, drying after soaking, full osmopriming process and germination after osmopriming.
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| Contributor(s) |
Kubala S, Garnczarska M, Wojtyla Ł, Clippe A, Kosmala A, Żmieńko A, Lutts S, Quinet M |
| Citation(s) |
25575995 |
| |
| Submission date |
Sep 24, 2014 |
| Last update date |
Feb 22, 2015 |
| Contact name |
Małgorzata Garnczarska |
| E-mail(s) |
garnczar@amu.edu.pl
|
| Organization name |
Adam Mickiewicz University
|
| Department |
Plant Physiology
|
| Street address |
Umultowska 89
|
| City |
Poznań |
| State/province |
wielkopolska |
| ZIP/Postal code |
61-614 |
| Country |
Poland |
| |
|
| Platforms (1) |
| GPL14582 |
Agilent-022520 Brassica napus 4x44K |
|
| Samples (8)
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| GSM1511455 |
rapeseed - osmopriming -soaking-replicat1 |
| GSM1511456 |
rapeseed - osmopriming -drying-replicat1 |
| GSM1511457 |
rapeseed - osmopriming -general-replicat1 |
| GSM1511458 |
rapeseed - post-priming germination-replicat1 |
| GSM1511459 |
rapeseed - osmopriming -soaking-replicat2 |
| GSM1511460 |
rapeseed - osmopriming -drying-replicat2 |
| GSM1511461 |
rapeseed - osmopriming -general-replicat2 |
| GSM1511462 |
rapeseed - post-priming germination-replicat2 |
|
| Relations |
| BioProject |
PRJNA261883 |
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