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| Status |
Public on Oct 15, 2015 |
| Title |
Transcript levels in CIRCADIAN CLOCK ASSOCIATED 1-overexpressing (CCA1-ox) Arabidopsis thaliana over a 24-hour diurnal cycle |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Circadian control of gene expression has been established in plants at the transcriptional level, but relatively little is known about circadian control of translation. We used polysome profiling to characterize regulation of transcription and translation over a 24-hour diurnal cycle in Arabidopsis, both in wild type and in plants with a disrupted clock due to constitutive overexpression of the CIRCADIAN CLOCK ASSOCIATED 1 gene (CCA1-ox, AGI AT2G46830).
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| Overall design |
10 day-old wild type and CCA1-ox (described in Cell. 1998 Jun 26;93(7):1207-17) Arabidopsis seedlings were harvested at 6am (Zeitgeber time ZT0), 12pm (ZT6), 6pm (ZT12), and 12am (ZT18), with 3 replicates for each time and genotype.
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| Contributor(s) |
von Arnim A, Ernest B, Missra A |
| Citation(s) |
26392078 |
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| Submission date |
Sep 30, 2014 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Albrecht G von Arnim |
| E-mail(s) |
vonarnim@utk.edu
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| Phone |
1-865-974-6206
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| Organization name |
The University of Tennessee
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| Department |
Biochemistry, Cellular and Molecular Biology
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| Lab |
von Arnim Lab
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| Street address |
M407 Walters Life Science Building
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| City |
Knoxville |
| State/province |
TN |
| ZIP/Postal code |
37996 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (12)
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| GSM1516673 |
CCA1-ox, 12am, total RNA, replicate 1 |
| GSM1516674 |
CCA1-ox, 6am, total RNA, replicate 2 |
| GSM1516675 |
CCA1-ox, 12pm, total RNA, replicate 2 |
| GSM1516676 |
CCA1-ox, 6pm, total RNA, replicate 2 |
| GSM1516677 |
CCA1-ox, 12am, total RNA, replicate 2 |
| GSM1516678 |
CCA1-ox, 6am, total RNA, replicate 3 |
| GSM1516679 |
CCA1-ox, 12pm, total RNA, replicate 3 |
| GSM1516680 |
CCA1-ox, 6pm, total RNA, replicate 3 |
| GSM1516681 |
CCA1-ox, 12am, total RNA, replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE61899 |
Polysome profiling in wild type and CIRCADIAN CLOCK ASSOCIATED 1-overexpressing (CCA1-ox) Arabidopsis thaliana over a 24-hour diurnal cycle |
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| Relations |
| BioProject |
PRJNA262638 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE61896_RAW.tar |
24.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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