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Series GSE62101 Query DataSets for GSE62101
Status Public on Oct 07, 2014
Title aCGH data of Rb/p53 deficient mammary tumors
Organism Mus musculus
Experiment type Genome variation profiling by genome tiling array
Summary To investigate the impact of combined Rb and p53 loss in mammary tumorigenesis, we used transgenic and viral approaches to delete Rb and p53 floxed alleles specifically in the mouse mammary epithelium. Although MMTV-Cre (NLST) targets stem/bi-potent progenitors in the mammary gland, a subset of MMTV-Cre:Rbf/f;p53f/f mice developed non-mammary tumors. Thus, freshly isolated primary mammary epithelial cells from these animals were transplanted into the mammary fat pads of immunodeficient mice and monitored for tumor formation. In addition, primary MECs were isolated from Cre-negative Rbf/f;p53f/f mice, infected with Ad-Cre followed by orthotopic transplantation. In all these cases, resulting tumors shared similar spindle-shape histology, expressed high levels of vimentin, a mesenchymal marker, but not E-cadherin, a luminal marker, and were classified as adeno-sacrcomatoid/spindle-cell/mesenchymal-like breast cancer. We used aCGH to detect copy number alterations associated with Rb/p53 deletion.
 
Overall design Tumor DNAs from MMTV-Cre: Rbf/f;p53f/f and Ad-Cre: Rbf/f;p53f/f conditional mutant mice are being compared to pooled tail DNAs in order to identify common alterations associated with Rb/p53 deficient tumorigenesis
 
Contributor(s) Jones R, Zacksenhaus E
Citation(s) 27571409
Submission date Oct 06, 2014
Last update date Feb 27, 2019
Contact name Robert Jones
E-mail(s) rjones12@uoguelph.ca
Organization name University of Guelph
Department Biomedical Sciences
Street address 50 Stone Rd
City Guelph
ZIP/Postal code N1G 2W1
Country Canada
 
Platforms (1)
GPL10448 Agilent-027414 SurePrint G3 Mouse CGH Microarray 1x1M
Samples (9)
GSM1519647 MMTV-Cre:Rbp53 primary tumor MCRP-1175
GSM1519648 MMTV-Cre:Rbp53 primary tumor MCRP-1241
GSM1519649 MMTV-Cre:Rbp53 primary tumor TMCRP-1
Relations
BioProject PRJNA263195

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62101_RAW.tar 903.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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