|
| Status |
Public on Jan 12, 2015 |
| Title |
Transcriptome Analysis of the Basal Chordate Botryllus schlosseri Reveals a Set of Genes Associated with Fertility |
| Organism |
Botryllus schlosseri |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Background: Gonad differentiation is an essential function for all sexually reproducing species, and many aspects of these developmental processes are highly conserved among the metazoa. The colonial ascidian, Botryllus schlosseri is a chordate model organism which offers two unique traits that can be utilized to characterize the genes underlying germline development: a colonial life history and variable fertility. These properties allow individual genotypes to be isolated at different stages of fertility and gene expression can be characterized comprehensively. Results: Here we characterized the transcriptome of both fertile and infertile colonies throughout blastogenesis (asexual development) using differential expression analysis. We identified genes (as few as 7 and as many as 647) regulating fertility in Botryllus at each stage of blastogenesis. Several of these genes appear to drive gonad maturation, as they are expressed by follicle cells surrounding both testis and oocyte precursors. Spatial and temporal expression of differentially expressed genes was analyzed by in situ hybridization, confirming expression in developing gonads. Conclusion: We have identified several genes expressed in developing and mature gonads in B. schlosseri. Analysis of genes upregulated in fertile animals suggests a high level of conservation of the mechanisms regulating fertility between basal chordates and vertebrates.
|
| |
|
| Overall design |
mRNA profiles of seven infertile stages from infertile Botryllus schlosseri colonies (each in triplicates) and seven stages of fertile Botryllus schlosseri colonies (each in triplicates) using Illumina deep sequencing and analyzed for differential expression between each stage.
|
| |
|
| Contributor(s) |
Rodriguez D, Sanders EN, Farell K, Langenbacher AD, Taketa DA, Hopper MR, Kennedy M, Gracey A, De Tomaso AW |
| Citation(s) |
25542255 |
| Submission date |
Oct 06, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Delany Rodriguez |
| E-mail(s) |
dr.delanyrodriguez@gmail.com
|
| Organization name |
University of California Santa Barbara
|
| Department |
Molecular Cellular and Developmental Biology
|
| Lab |
De Tomaso Lab
|
| Street address |
Biological Science Building RM1201 University of California Santa Barbara
|
| City |
Santa Barbara |
| State/province |
CA |
| ZIP/Postal code |
93106 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19268 |
Illumina HiSeq 2000 (Botryllus schlosseri) |
|
| Samples (42)
|
|
| Relations |
| BioProject |
PRJNA263209 |
| SRA |
SRP048687 |
Less...
More...