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| Status |
Public on Feb 13, 2015 |
| Title |
HuR- dependent regulation of mRNA splicing is essential for the B cell antibody response [Ribo-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Post-transcriptional regulation of mRNA by the RNA binding protein HuR is required in B cells for the germinal centre reaction and for the production of class-switched antibodies in response to T-independent antigens. Transcriptome-wide examination of RNA isoforms, abundance and translation in HuR-deficient B cells, together with direct measurements of HuR-RNA interaction, revealed that HuR-dependent mRNA splicing affects hundreds of transcripts including the dihydrolipoyl succinyltransferase (Dlst), a subunit of the aketoglutaratedehydrogenase (aKGDH) enzyme. In the absence of HuR, defective mitochondrial metabolism results in high levels of reactive oxygen species and B cell death. Our study shows how post-transcriptional processes control the balance of energy metabolism required for B cell proliferation and differentiation.
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| Overall design |
Sequencing analysis of ribosome protected RNA fragments in ex vivo or LPS-activated splenic B cells was performed using ARTseq™ Ribosome Profiling Kit - Mammalian (Epicentre, RPHMR12126) and Illumina platform. Splenic B cells come from HuRf/f control or HuR cKO mice. 4-5 biological replicates per genotype and condition.
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| Contributor(s) |
Diaz-Muñoz MD, Bell SE, Fairfax K, Monzon-Casanova E, Cunningham AF, Gonzalez-Porta M, Andrews SR, Bunik VI, Zarnack K, Curk T, Kontoyiannis DL, Ule J, Turner M |
| Citation(s) |
25706746 |
| |
| Submission date |
Oct 07, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Felix Krueger |
| E-mail(s) |
fkrueger@altoslabs.com
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| Organization name |
Altos Labs
|
| Department |
Bioinformatics
|
| Street address |
Granta Park
|
| City |
Cambridge |
| ZIP/Postal code |
CB21 6GP |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE62148 |
HuR- dependent regulation of mRNA splicing is essential for the B cell antibody response |
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| Relations |
| BioProject |
PRJNA263261 |
| SRA |
SRP048708 |
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