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Series GSE62176 Query DataSets for GSE62176
Status Public on Oct 17, 2014
Title Analysis of antibody production in a mouse model of Sjögren’s Syndrome
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus
Sample organism Mus musculus
Experiment type Protein profiling by protein array
Summary Auto-antigen array data show IgM from salivary B cells is enriched for anti-nuclear antigen specificity, including Ro (SSA) and La (SSB).
 
Overall design B cells were sort purified from spleen, cervical lymph nodes and submandibular salivary tissue (SMG) tissue of Id3-/- mice. We also sort purified B cells from spleen and cervical lymph nodes of age and gender matched C57BL/6 mice. Cells (1x10^5 /well) were cultured in 5% RPMI 1640, stimulated with LPS (25 μg/mL) for 6 days, and supernatants harvested. Sera from SS mice were harvested by cardiac puncture immediately following euthanasia. Blood was allowed to clot for two hours and centrifuged at 2,000 g for twenty minutes. Serum was removed and stored at -20°C until use. These undiluted supernatant samples and sera were used to perform the autoantigen array.
 
Contributor(s) Kramer JM, Holodick NE, Vizconde T, Raman I, Yan M, Li Q, Gaile DP, Rothstein TL
Citation(s) 26382297
Submission date Oct 08, 2014
Last update date Mar 03, 2016
Contact name yun lian
E-mail(s) yun.lian@utsouthwestern.edu
Organization name UT Southwestern Medical Center
Street address 5323 Harry Hines Blvd.
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
 
Platforms (1)
GPL19273 Autoantigen Microarray
Samples (74)
GSM1520995 Id3 serum 1 (IgG)
GSM1520996 Id3 serum 2 (IgG)
GSM1520997 B6 serum 1 (IgG)
Relations
BioProject PRJNA264079

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62176_13samplesIgG_no_normalized.txt.gz 3.3 Kb (ftp)(http) TXT
GSE62176_13samplesIgM_no_normalized.txt.gz 4.1 Kb (ftp)(http) TXT
GSE62176_24samplesIgG_no_normalized.txt.gz 3.8 Kb (ftp)(http) TXT
GSE62176_24samplesIgm_no_normalized.txt.gz 6.0 Kb (ftp)(http) TXT
GSE62176_PBS_IgG_raw_data.txt.gz 1002 b (ftp)(http) TXT
GSE62176_PBS_IgM_raw_data.txt.gz 1.0 Kb (ftp)(http) TXT
Processed data included within Sample table

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