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| Status |
Public on Oct 10, 2014 |
| Title |
Elevated carbon dioxide alters the interaction between Eucalyptus grandis and Pisolithus microcarpus through a complex shift in the root transcriptome |
| Organisms |
Eucalyptus grandis; Pisolithus microcarpus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Illumina HiSeq technology was used to generate mRNA profiles from in vitro Eucalyptus grandis roots interacting with two different Pisolithus microcarpus strains (SI-9 and SI-12) and under two different CO2 concentrations (400 and 650 ppm) . Control roots or ectomycorrhizal root tips were harvested after 1 month and used for RNA extraction. Paired-end (2X150bp) reads were generated and aligned to Eucalyptus grandis transcripts (http://www.phytozome.net/; primarytranscripts only) using CLC Genomics Workbench 6.
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| Overall design |
mRNA profiles from in vitro Eucalyptus grandis roots interacting with two different Pisolithus microcarpus strains (SI-9 and SI-12) and under two different CO2 concentrations (400 and 650 ppm) were generated by paired-end (2X110bp) Illumina HiSeq1500 sequencing. Three biological replicates were sequenced for mycorrhizal samples, five for CO2 650ppm control roots and six for CO2 400ppm control roots.
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| Contributor(s) |
Plett JM, Kohler A, Janitz C, Khachane A, Martin F, Anderson IC |
| Citation(s) |
25377589 |
| Submission date |
Oct 09, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Annegret Kohler |
| E-mail(s) |
annegret.kohler@inrae.fr
|
| Phone |
+33 (0)383 394072
|
| Organization name |
INRAE
|
| Department |
UMR 1136
|
| Lab |
Interactions Arbres/Micro-organismes
|
| Street address |
Centre INRAE Grand Est Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
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| Platforms (2) |
| GPL19281 |
Illumina HiSeq 1500 (Eucalyptus grandis) |
| GPL19282 |
Illumina HiSeq 1500 (Eucalyptus grandis; Pisolithus microcarpus) |
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| Samples (23)
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| Relations |
| BioProject |
PRJNA263510 |
| SRA |
SRP048810 |
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