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| Status |
Public on Nov 21, 2014 |
| Title |
Convergent evolution of complex regulatory landscapes and pleiotropy at Hox loci (4C-seq) |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Hox genes are required during the morphogenesis of both vertebrate digits and external genitals.We investigated whether transcription in such distinct contexts involves a shared enhancer-containing landscape. We show that the same regulatory topology is used, yet with some tissue-specific enhancer-promoter interactions, suggesting the hijacking of a regulatory backbone from one context to the other. In addition, comparable organizations are observed at both HoxA and HoxD clusters, which separated through genome duplication in an ancestral invertebrate animal.We propose that this convergent regulatory evolution was triggered by the pre-existence of some chromatin architecture, thus facilitating the subsequent recruitment of the appropriate transcription factors. Such regulatory topologies may have both favored and constrained the evolution of pleiotropic developmental loci in vertebrates.
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| Overall design |
Circular Chromosome Conformation Capture (4C-seq) samples from mouse digit cells and mouse genital tubercle cells performed as previously described (Noordermeer et al. 2011)
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| Contributor(s) |
Lonfat N, Montavon T, Darbellay F, Gitto S, Duboule D |
| Citation(s) |
25414315 |
| |
| Submission date |
Oct 10, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicolas Lonfat |
| Organization name |
EPFL - Ecole Polytechnique Fédérale de Lausanne
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| Department |
School of Life Sciences
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| Lab |
Laboratory of developmental genomics
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| Street address |
EPFL-SV-ISREC-UPDUB- Station 19
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| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE62269 |
Convergent evolution of complex regulatory landscapes and pleiotropy at Hox loci |
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| Relations |
| BioProject |
PRJNA263615 |
| SRA |
SRP048835 |
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