|
| Status |
Public on Oct 30, 2014 |
| Title |
An Ezh2 and Ezh1 dual inhibitor, UNC1999, re-shapes the landscape of H3K27me3 versus H3K27ac in MLL-AF9-transformed murine leukemia |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Ezh2 and EZH1 are histone H3 lysine 27 (H3K27)-specific methyltransferases. Their hyperactive mutations and overexpression were found in cancer including various hematological malignancies. UNC1999 is a highly selective inhibitor for both enzymes. It suppresses H3K27 tri- and di-methylation globally and inhibits growth of MLL-rearranged acute leukemia. Here we performed ChIP-Seq to profile how UNC1999 affects distribution of H3K27me3 and its antagonizing H3K27ac in MLL-AF9-immortalized leukemia cells. We also performed ChIP-seq of SUZ12, an essential common cofactor of EZH2 and EZH1 following compound treatments.
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| |
|
| Overall design |
We treated MLL-AF9 transformed murine leukemia cells with DMSO, UNC1999 or UNC2400 (an inactive analog compound of UNC1999). Cells were then collected and used for ChIP-Sequencing of Input, H3K27me3, SUZ12, and H3K27ac.
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| |
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| Contributor(s) |
On D, Bowen XU, Wang GG |
| Citation(s) |
25395428 |
| |
| Submission date |
Oct 16, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Bowen Xu |
| E-mail(s) |
bowenx@email.unc.edu
|
| Phone |
919-966-5953
|
| Organization name |
UNC-Chapel Hill
|
| Department |
Biochemistry and Biophysics
|
| Lab |
Wang
|
| Street address |
450 West Drive, CB7295, Lineberger Cancer Center, GREG WANG Lab, RM 31-331
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (11)
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| Relations |
| BioProject |
PRJNA264095 |
| SRA |
SRP048995 |
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