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| Status |
Public on May 19, 2017 |
| Title |
Recruitment of the NineTeen Complex to the activated spliceosome requires AtPRMT5 |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we analyzed the pre-mRNA splicing status in wild-type Col, atprmt5 mutants, and two atprmt5 suppressors (m90 and s215). RNA-seq analyses revealed that m90 and s215 can partially rescue the pre-mRNA splicing defects in atprmt5 mutants. Further study showed that m90 and s215 were identified in the highly conserved pre-mRNA splicing factor 8 (AtPrp8) and demonstrated that Prp19C/NTC complex failed to be assembled into U5 snRNP to form activated spliceosome in atprmt5 mutants, while restored in the suppressor. Our findings uncover a key molecular mechanism for AtPRMT5 in the regulation of pre-mRNA splicing.
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| |
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| Overall design |
Investigate the pre-mRNA splicing in the suppressors of atprmt5 mutants
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| |
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| Contributor(s) |
Deng X, Lu T, Wang L, Gu L, Liu C, Kong X, Cao X |
| Citation(s) |
27114555 |
| |
| Submission date |
Oct 22, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
xiaofeng cao |
| E-mail(s) |
xfcao@genetics.ac.cn
|
| Phone |
86-10-64869203
|
| Organization name |
Institute of Genetics and Developmental Biology
|
| Department |
State Key Laboratory of Plant Genomics
|
| Street address |
West Lincui Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA264533 |
| SRA |
SRP049166 |
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