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Series GSE6302 Query DataSets for GSE6302
Status Public on Feb 28, 2007
Title ADH1 deletion cells upon growth on alternative carbon sources; Steady-state grown cells upon environmental perturbations
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Cells must adjust their gene expression in order to compete in a constantly changing environment. Two alternative strategies could in principle ensure optimal coordination of gene expression with physiological requirement. First, the internal physiological state itself could feedback to regulated gene expression. Second, the expected physiological state could be inferred from the external environment, using evolutionary-tuned signaling pathways. Coordination of ribosomal biogenesis with the requirement for protein synthesis appears to be particularly important, since cells devote a large fraction of their biosynthetic capacity for ribosomal biogenesis. To define the relative importance of internal vs. external sensing to the regulation of ribosomal biogenesis gene expression, we subjected S. cerevisiae cells to conditions which decoupled the actual vs. environmentally-expected growth rate. Gene expression followed the environmental signal according to the expected, but not the actual, growth rate. Simultaneous monitoring of gene expression and growth rate in chemostat-grown cultures further confirmed that ribosome biogenesis genes responded rapidly to changes in the environments but were oblivious to longer-term changes in growth rate. Our results suggest that the capacity to anticipate and prepare for environmental changes presented a major selection force during yeast evolution.
Keywords: Saccharomyces_Cerevisiae, Stress response, ADH1 deletion, time courses, chemostat
 
Overall design Experiment 1: ADH1 deletion cells, which grow faster on glycerol rather then glucose medium, were grown in both glucose and glycerol medium. The cells expression on glycerol is compared to their expression glucose (5 microarrays). Experiment 2: Time course of ADH1 deletion cells upon growth on glycerol and on glucose (7 microarrays). Experiment 3: Response of steady state grown cells to environmental perturbations. Cells were grown in glucose or histidine limited chemostats and reached steady state. The cells were then subjected to perturbations such as DTT, heat shock, NaCl, Clotrimazole, H2O2, and addition of limiting factors (histidine and glucose, respectively). Overall we have examined 10 timecourses with 83 microarrays.
 
Contributor(s) Levy S, Ihmels J, Carmi M, Weinberger A, Friedlander G, Barkai N
Citation(s) 17327914
Submission date Nov 16, 2006
Last update date Mar 17, 2012
Contact name sagi levy
E-mail(s) sagi.levy@weizmann.ac.il
Phone 972-8-9342201
URL http://barkai-serv.weizmann.ac.il/GroupPage/
Organization name Weizmann Institute of Science
Department Molecular Genetics and Physics of Complex Systems
Lab Naama Barkai
Street address Hertzel
City Rehovot
ZIP/Postal code 76100
Country Israel
 
Platforms (1)
GPL4567 UHN Yeast 6.4kv6
Samples (95)
GSM144760 WT BY4741- glycerol vs. glucose at log phase- #1
GSM144761 WT BY4741- glycerol vs. glucose at log phase- #2
GSM144762 ADH1 deletion mutant- glycerol vs. glucose at log phase- #1
Relations
BioProject PRJNA100503

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6302_RAW.tar 321.4 Mb (http)(custom) TAR (of XLS)

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