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| Status |
Public on Oct 19, 2005 |
| Title |
Auxin mediated gene expression in WT and arf2-6 mutant |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Global gene expression data from 7-day old light-grown liquid cultured seedlings treated with or without auxin (5µM IAA) for 2 h. Columbia (WT) and Auxin response factor 2 (ARF2) T-DNA insertion mutant (arf2-6 ) were used for this study. Each experimental condition has three true replicates for a total of 12 hybridizations.
Data analysis:
Affymetrix GeneChip Microarray Suite version 5.0 software was used to obtain signal values for individual genes. The data files containing the probe level intensities (cell files) were used for background correction and normalization using the log2 scale robust multi-array analysis (RMA) procedure (Irizarry et al., 2003). The “R” environment (Ihaka and Gentleman, 1996) was used for running the RMA program. Data analysis and statistical extraction were performed using log2 converted expression intensity data within Microsoft Excel 98. Based on preliminary analysis, a hybridization signal less than 5.64385619 (= log2 50) was considered as background; all signals less than 5.64385619 were converted to 5.64385619 prior to further analysis.
Keywords = Auxin
Keywords = Auxin response factor
Keywords: other
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| Contributor(s) |
Okushima Y, Theologis A |
| Citation(s) |
15960614 |
| Submission date |
Sep 04, 2003 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yoko Okushima |
| E-mail(s) |
okushima@bs.naist.jp
|
| Phone |
510-559-5921
|
| Fax |
510-559-5678
|
| Organization name |
Plant Gene Expression Center, U. C. Berkeley
|
| Department |
Plant and Microbial Biology
|
| Lab |
Athanasios Theologis Lab.
|
| Street address |
800 Buchanan St.
|
| City |
Albany |
| State/province |
CA |
| ZIP/Postal code |
94710 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE630 |
Auxin-mediated gene expression |
|
| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA89755 |
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