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Series GSE63217 Query DataSets for GSE63217
Status Public on Mar 27, 2018
Title Inhibitors of LSD1 target demethylase-independent activity to induce differentiation in acute myeloid leukemia [RNA-Seq experiments]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To determine whether changes in histone modifications directly correlate with changes in transcription, THP1 AML cells were treated with a potent and selective LSD1 inhibitor (OG86) and then subjected to concomitant RNA sequencing (RNAseq) and ChIP sequencing (ChIPseq) for monomethyl-, dimethyl- and trimethyl histone H3 modifications.
 
Overall design THP1 AML cells were treated with 250nM OG86 or DMSO vehicle for 24 hours in semi-solid culture. Two technical replicates were analysed for each sample.
 
Contributor(s) Somervaille T
Citation(s) 29590629
Submission date Nov 12, 2014
Last update date May 15, 2019
Contact name Tim C Somervaille
E-mail(s) tim.somervaille@cruk.manchester.ac.uk
Organization name Cancer Research UK Manchester Institute
Lab Leukaemia Biology Laboratory
Street address Wilmslow Road
City Manchester
State/province Lancashire
ZIP/Postal code M20 4BX
Country United Kingdom
 
Platforms (1)
GPL16558 AB 5500 Genetic Analyzer (Homo sapiens)
Samples (4)
GSM1543972 THP1_DMSO_replicate1
GSM1543973 THP1_DMSO_replicate2
GSM1543974 THP1_OG86_replicate1
This SubSeries is part of SuperSeries:
GSE63222 Inhibitors of LSD1 target demethylase-independent activity to induce differentiation in acute myeloid leukemia
Relations
BioProject PRJNA267008
SRA SRP049731

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63217_Processed_data_file.txt.gz 389.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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