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Status |
Public on Mar 27, 2018 |
Title |
Inhibitors of LSD1 target demethylase-independent activity to induce differentiation in acute myeloid leukemia [RNA-Seq experiments] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To determine whether changes in histone modifications directly correlate with changes in transcription, THP1 AML cells were treated with a potent and selective LSD1 inhibitor (OG86) and then subjected to concomitant RNA sequencing (RNAseq) and ChIP sequencing (ChIPseq) for monomethyl-, dimethyl- and trimethyl histone H3 modifications.
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Overall design |
THP1 AML cells were treated with 250nM OG86 or DMSO vehicle for 24 hours in semi-solid culture. Two technical replicates were analysed for each sample.
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Contributor(s) |
Somervaille T |
Citation(s) |
29590629 |
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Submission date |
Nov 12, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Tim C Somervaille |
E-mail(s) |
tim.somervaille@cruk.manchester.ac.uk
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Organization name |
Cancer Research UK Manchester Institute
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Lab |
Leukaemia Biology Laboratory
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Street address |
Wilmslow Road
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City |
Manchester |
State/province |
Lancashire |
ZIP/Postal code |
M20 4BX |
Country |
United Kingdom |
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Platforms (1) |
GPL16558 |
AB 5500 Genetic Analyzer (Homo sapiens) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE63222 |
Inhibitors of LSD1 target demethylase-independent activity to induce differentiation in acute myeloid leukemia |
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Relations |
BioProject |
PRJNA267008 |
SRA |
SRP049731 |