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Series GSE63224 Query DataSets for GSE63224
Status Public on Oct 27, 2016
Title Comprehensive transcriptome analysis of phytohormone pathway genes and miRNAs in ABA initiated strawberry receptacle ripening
Organism Fragaria x ananassa
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Other
Summary We used Illumina sequencing to investigate the global transcriptomic expression of hormonal pathway genes in ABA initiated strawberry receptacle ripening. Expression profiles of hormone synthetic and signaling genes further demonstrated the positive roles of ABA and GA, and the negative role of auxin in receptacle ripening. We also evaluated the transcript profiling of ethylene and JA pathway genes, and the results suggested that both ethylene and JA participated in receptacle ripening. Furthermore, two novel miRNAs and three conserved miRNAs were identified and validated to target genes in ABA and auxin pathways, respectively. Our analyses reveal the molecular mechanism of hormonal regulation during strawberry receptacle ripening. The data also provide an abundant of genetic information for molecular manipulation on non-climacteric fruit ripening.
 
Overall design Sample 1: CK0 (Strawberry fruit two weeks after athesis treated with water, set as day 0); Sample 2: CK5 (fruit treated with water on day 5); Sample 3: CK8 (fruit treated with water on day 8); Sample 4: ABA5 (fruit treated with ABA on day 5); Sample 5: ABA8 (fruit treated with ABA on day 5); Sample 6: NDGA5 (fruit treated with water on day 5); Sample 7: NDGA8 (fruit treated with NDGA on day 8).
 
Contributor(s) Li D, Luo Z
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 12, 2014
Last update date May 15, 2019
Contact name Dongdong Li
E-mail(s) leedong2@126.com
Phone 86+0571+13732214067
Organization name Zhejiang University
Department Food Science and Nutrition
Lab 406
Street address Yuhangtang Road
City Hangzhou
State/province Zhejiang
ZIP/Postal code 310058
Country China
 
Platforms (1)
GPL15080 Illumina HiSeq 2000 (Fragaria x ananassa)
Samples (21)
GSM1544010 CK0_transcriptome
GSM1544011 CK5_transcriptome
GSM1544012 CK8_transcriptome
Relations
BioProject PRJNA267010
SRA SRP049736

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63224_All_Expressed_miRNA.xlsx 625.1 Kb (ftp)(http) XLSX
GSE63224_All_unigene_expression.txt.gz 7.3 Mb (ftp)(http) TXT
GSE63224_Degradome_result.xlsx 677.8 Kb (ftp)(http) XLSX
GSE63224_Trinity_unique_gene_sequence.fasta.gz 36.4 Mb (ftp)(http) FASTA
GSE63224_gene_annotation.txt.gz 3.8 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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