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Status |
Public on Jan 30, 2015 |
Title |
Bioinformatics approaches for viral metagenomics in plants using short RNAs : model case of study and application to a Cicer arietinum population |
Organism |
Cicer arietinum |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
Summary |
Size fractionated small RNA from total RNA extracts of Cicer arietinum leaves and from Nicotiana benthamiana infected by Cymbidium ringspot virus were mixed in a ratio of 1000 to 1 in amount, respectively. The RNA was ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Illumina high throughput pyrosequencing. The kit used is TrueSeq Small RNA kit Please see www.illumina.com for details of the sequencing technology.
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Overall design |
Short RNA fractionation and characterization
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Contributor(s) |
Pantaleo V |
Citation(s) |
25674078 |
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Submission date |
Nov 17, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Vitantonio Pantaleo |
E-mail(s) |
vitantonio.pantaleo@cnr.it
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Phone |
00390805442935
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Organization name |
Institute for Sustainable Plant Protection
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Lab |
Research Unit of Bari (ex IVV-CNR)
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Street address |
Via Amendola 165/a
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City |
Bari |
ZIP/Postal code |
70126 |
Country |
Italy |
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Platforms (1) |
GPL19428 |
Illumina HiSeq 2500 (Cicer arietinum) |
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Samples (1) |
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Relations |
BioProject |
PRJNA267652 |
SRA |
SRP049984 |