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Series GSE63378 Query DataSets for GSE63378
Status Public on Jan 30, 2015
Title Bioinformatics approaches for viral metagenomics in plants using short RNAs : model case of study and application to a Cicer arietinum population
Organism Cicer arietinum
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Size fractionated small RNA from total RNA extracts of Cicer arietinum leaves and from Nicotiana benthamiana infected by Cymbidium ringspot virus were mixed in a ratio of 1000 to 1 in amount, respectively. The RNA was ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Illumina high throughput pyrosequencing. The kit used is TrueSeq Small RNA kit Please see www.illumina.com for details of the sequencing technology.
 
Overall design Short RNA fractionation and characterization
 
Contributor(s) Pantaleo V
Citation(s) 25674078
Submission date Nov 17, 2014
Last update date May 15, 2019
Contact name Vitantonio Pantaleo
E-mail(s) vitantonio.pantaleo@cnr.it
Phone 00390805442935
Organization name Institute for Sustainable Plant Protection
Lab Research Unit of Bari (ex IVV-CNR)
Street address Via Amendola 165/a
City Bari
ZIP/Postal code 70126
Country Italy
 
Platforms (1)
GPL19428 Illumina HiSeq 2500 (Cicer arietinum)
Samples (1)
GSM1547707 RNA extracted from leaves of plants
Relations
BioProject PRJNA267652
SRA SRP049984

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63378_RAW.tar 730.0 Kb (http)(custom) TAR (of TAB)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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