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Series GSE63383 Query DataSets for GSE63383
Status Public on Jan 31, 2015
Title Expression data from asthmatic and healthy airway smooth muscle cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Persistent severe asthma is associated with hyper-contractile airways and structural changes in the airway wall, including an increased airway smooth muscle (ASM) mass. This study used gene expression profiles from asthmatic and healthy airway smooth muscle cells grown in culture to identify novel receptors and pathways that potentially contributed to asthma pathogenesis.
We used microarrays to compare the gene expression between asthmatic and healthy airway smooth muscle cells to understand the underlying pathway contributing the differences in cellular phenotypes
 
Overall design Asthmatic airway smooth muscle cells (ASMC) are intrinsically different and have a differential transcriptional response to pro-fibrotic, pro-proliferation and pro-inflammatory stimuli than ASMC from healthy patients. We sought to identify genes that are differentially expressed between asthmatic and healthy ASMC under various stimulations which mimic the asthmatic airways. To this end, we obtained human ASMC from bronchial biopsies and explanted lungs from doctor diagnosed asthmatic patients (n=3) and healthy controls (n=3). The ASMC were then grown in culture and treated with pro-fibrotic (Transforming growth factor beta (TGFβ)), pro-proliferation (Fetal Bovine Serum (FBS)) and pro-inflammatory stimuli (Interleukin-1 beta (IL-1β)) for 8 hours. Gene expression was then evaluated using Affymetrix Human Gene 1.0ST arrays.
 
Contributor(s) Faiz A, Oliver BG, Black JL, Burgess JK
Citation(s) 27325752
Submission date Nov 17, 2014
Last update date Jul 26, 2018
Contact name Alen Faiz
E-mail(s) a.faiz@umcg.nl
Organization name Woolcock Institute of Medical Research
Lab Cell Biology
Street address 431 Glebe Point Rd
City Sydney
State/province NSW
ZIP/Postal code 2037
Country Australia
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (24)
GSM1547794 Healthy airway smooth muscle following treatment with 10% FBS for 8H, patient 1
GSM1547795 Healthy airway smooth muscle following treatment with IL1B (10ng/ml) for 8H, patient 2
GSM1547796 Healthy airway smooth muscle following treatment with TGFB (10ng/ml) for 8H, patient 2
Relations
BioProject PRJNA267606

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63383_RAW.tar 101.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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