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| Status |
Public on Jul 20, 2015 |
| Title |
Characterization of mouse histone H3 variants |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The selective incorporation of appropriate histone variants into chromatin is critical for the regulation of genome function. Although many histone variants have been identified, a complete list has not been compiled. We screened human and mouse genomes by in silico hybridization using canonical histone sequences, and found 14 uncharacterized H3-related genes in mouse. Most were similar to the H3.3 variant; their 3′ untranslated regions lacked a stem-loop structure but did have a poly- adenylation signal. Transcriptome analysis revealed tissue-specific expression of these variants. When expressed as GFP-tagged versions in mouse C2C12 cells, some variants were stably incorporated into chromatin and the genome distributions of most variants were similar to that of H3.3. Over-expression of some variants altered gene expression patterns in differentiated C2C12 cells, suggesting that these new H3 variants have specific and distinct functions.
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| Overall design |
RNA-Seq and ChIP-Seq for GFP fused Histone H3 variants of C2C12 cells in growth or differentiated conditions; 3'-seq analysis for C2C12 cells and mouse tissues
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| Contributor(s) |
Ohkawa Y, Maehara K, Harada A |
| Citation(s) |
26388943 |
| BioProject |
PRJDB3075 |
| |
| Submission date |
Dec 05, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Yasuyuki Ohkawa |
| E-mail(s) |
yohkawa@bioreg.kyushu-u.ac.jp
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| Organization name |
Kyushu University
|
| Department |
Medical Institute of Bioregulation, Medical Institute of Bioregulation
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| Street address |
3-1-1 Maidashi, Higashi-ku, Fukuoka
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| City |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (66)
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