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| Status |
Public on Apr 16, 2015 |
| Title |
Validation of EZH2 targets in GSCs treated with shNT (control), shMELK, shFOXM1, and EZH2 overexpression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We demonstrate that the catalytic subunit of Polycomb Repressive Complex 2, EZH2, is targeted by the MELK-FOXM1 complex, which in turn promotes resistance to radiation in GSCs. Clinically, EZH2 and MELK are co-expressed in GBM and significantly induced in post-irradiation recurrent tumors whose expression inversely correlated with patient prognosis. Through gain-and loss-of-function study, our data show that MELK or FOXM1 contributes on GSC radioresistance by regulation of EZH2.
We used microarrays to validate EZH2 target gene expression.
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| Overall design |
GSCs were treated with shNT (control), shMELK, shFOXM1, and EZH2 overexpression. Total RNA was isolated using the Qiagen RNeasy kit (Qiagen).
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| Contributor(s) |
Nakano I, Kim S |
| Citation(s) |
25601206 |
| Submission date |
Dec 08, 2014 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Ichiro Nakano |
| E-mail(s) |
ichiro.nakano@osumc.edu
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| Phone |
614-290-0358
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| Organization name |
Ohio State University
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| Street address |
400w 12th, wisemanhall
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| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43215 |
| Country |
USA |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA269617 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE63963_RAW.tar |
127.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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