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Series GSE640 Query DataSets for GSE640
Status Public on Oct 02, 2003
Title Spermatogenesis
Organism Mus musculus
Experiment type Expression profiling by array
Summary A multitude of genes expressed solely in meiotic or postmeiotic spermatogenic cells offers a myriad of contraceptive targets.

Understanding mammalian spermatozoan development and the events surrounding fertilization has grown slowly, in part because of uncertainty about the number and identity of the cellular components involved. Determination of those transcripts expressed specifically by germ cells should provide an inclusive list of probable critical proteins. Here, total mouse testis transcript profiles were trimmed of transcripts found in cultures enriched in Sertoli or interstitial cells to yield a germ cell-enriched transcript profile. Monitoring of changes of this profile in the developing testis identified 1,652 genes whose transcript abundance increased markedly coincident with the onset of meiosis. Remarkably, 351 of these genes ( approximately 20%) appear to be expressed only in the male germline. Germ cell-specific transcripts are much less common earlier in testis development. Further analysis of the UniGene EST database coupled with quantitative PCR indicates that approximately 4% of the mouse genome is dedicated to expression in postmeiotic male germ cells. Most or many of the protein products of these transcripts are probably retained in mature spermatozoa. Targeted disruption of 19 of these genes has indicated that a majority have roles critical for normal fertility. Thus, we find an astonishing number of genes expressed specifically by male germ cells late in development. This extensive group provides a plethora of potential targets for germ cell-directed contraception and a staggering number of candidate proteins that could be critical for fertilization.
Keywords: other
 
 
Contributor(s) Schultz N, Hamra FK, Garbers DL, Schultz N, Garbers DL
Citation(s) 14526100
Submission date Sep 09, 2003
Last update date Feb 18, 2018
Contact name Nikolaus Schultz
E-mail(s) schultz@cbio.mskcc.org
Phone 646-888-2604
Organization name Memorial Sloan-Kettering Cancer Center
Department Computational Biology Center
Street address 1275 York Ave, Box 460
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (3)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
GPL82 [MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array
GPL83 [MG_U74Cv2] Affymetrix Murine Genome U74 Version 2 Array
Samples (51)
GSM9865 mouse_testis_adult_GPL81
GSM9866 mouse_testis_adult_GPL82
GSM9867 mouse_testis_adult_GPL83
Relations
BioProject PRJNA87555

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Supplementary file Size Download File type/resource
GSE640_RAW.tar 143.0 Mb (http)(custom) TAR (of CEL)

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