|
| Status |
Public on Nov 27, 2007 |
| Title |
TNFalpha interferes with adaptation to starvation and activates pro-atherogenic processes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Pathenogenesis of atherosclerosis results from the interactions between disrupted lipid homeostasis and immune response, but the molecular bridges between the major players are still a matter of controversy. We performed a systemic study of the imflammatory cytokine tumor necrosis factor alpha (TNF alpha), a well established anorexia agent, in the livers of the mice exposed to 20h-cytokine/starvation. We show that treatment with TNF-alpha reverses adaptations to fasting. Moreover, it up-regulates cholesterol biosynthesis and down-regulates bile acids synthesis, which leads to disrupted cholesterol homeostasis and pro-atherogenic changes. Interestingly, we found that TNF alpha also interferes with gene regulation of the xenobiotic metabolism.
Keywords: treatment and diet effects
|
| |
|
| Overall design |
Mice have been either saline treated (nontreated group), saline treated and fasted for 20h (fasted group), and TNF-alpha treated (30 mikrograms per animal) and fasted for 20h (TNF-alpha group). 3 biological replicas from fasted and TNF-alpha groups were co-hybridized with pool of nontreated group. No dye-swaps were performed.
|
| |
|
| Contributor(s) |
Fon Tacer K, Juvan P, Rozman D |
| Citation(s) |
18261244 |
| |
| Submission date |
Dec 04, 2006 |
| Last update date |
Aug 31, 2012 |
| Contact name |
Peter Juvan |
| Phone |
+386 1 543 7595
|
| Organization name |
Faculty of Medicine, University of Ljubljana
|
| Department |
Institute of Biochemistry
|
| Lab |
Center for Functional Genomics and Bio-Chips
|
| Street address |
Zaloska 4
|
| City |
Ljubljana |
| ZIP/Postal code |
SI-1000 |
| Country |
Slovenia |
| |
|
| Platforms (1) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA99495 |
Less...
More...