|
| Status |
Public on Sep 23, 2015 |
| Title |
Functional Retinal Pigment Epithelium-like Cells from Human Fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The retinal pigment epithelium (RPE) provides vital support to photoreceptor cells and its dysfunction is associated with the onset and progression of age-related macular degeneration (AMD). Surgical provision of RPE cells may ameliorate AMD and thus it would be valuable to develop sources of patient-matched RPE cells for this application of regenerative medicine. We describe here the generation of functional RPE-like cells from fibroblasts that represent an important step toward that goal. We identified candidate master transcriptional regulators of RPEs using a novel computational method and then used these regulators to guide exploration of the transcriptional regulatory circuitry of RPE cells and to reprogram human fibroblasts into RPE-like cells. The RPE-like cells share key features with RPEs derived from healthy individuals, including morphology, gene expression and function, and thus represent a step toward the goal of generating patient-matched RPE cells for treatment of macular degeneration.
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| |
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| Overall design |
Expression analysis was performed on induced retinal pigment epithelium-like cells.
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| |
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| Contributor(s) |
D'Alessio A, Fan ZP, Lee TI, Young RA |
| Citation(s) |
26603904 |
| |
| Submission date |
Dec 16, 2014 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Richard A Young |
| E-mail(s) |
young_computation@wi.mit.edu
|
| Phone |
617-258-5219
|
| Organization name |
Whitehead Institute for Biomedical Research
|
| Lab |
Young Lab
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
| GPL16043 |
GeneChip® PrimeView™ Human Gene Expression Array (with External spike-in RNAs) |
|
| Samples (20)
|
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| Relations |
| BioProject |
PRJNA270548 |
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