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| Status |
Public on Mar 11, 2015 |
| Title |
Cell cycle-independent phospho-regulation of Fkh2 during hyphal growth regulates Candida albicans pathogenesis. |
| Organism |
Candida albicans |
| Experiment type |
Expression profiling by array
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| Summary |
The opportunistic human fungal pathogen, Candida albicans, undergoes morphological and transcriptional adaptation in the switch from commensalism to pathogenicity. Although previous gene-knockout studies have identified many factors involved in this transformation, it remains unclear how these factors are regulated to coordinate the switch. Investigating morphogenetic control by post-translational phosphorylation has generated important regulatory insights into this process, especially focusing on coordinated control by the cyclin-dependent kinase Cdc28. Here we have identified the Fkh2 transcription factor as a regulatory target of both Cdc28 and the cell wall biosynthesis kinase Cbk1, in a role distinct from its conserved function in cell cycle progression. Transcript profiling of Fkh2 deletion and non-phosphrylatable mutants in yeast and hypae revealed that the the hyphal-specific shift in the phosphorylation profile is required for the expression of genes involved in pathogenesis, host interaction and biofilm formation. We confirmed that these changes in gene expression resulted in corresponding defects in pathogenic processes
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| Overall design |
Two colour arrays comparing both non-phosphylatable Fkh2 and double deletion mutants to their respective wildtypes in both yeast and hyphal C. albicans.
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| Contributor(s) |
Sudbery IM, Greig JA, Richardson JP, Naglik J, Wang Y, Sudbery PE |
| Citation(s) |
25617770 |
| |
| Submission date |
Dec 19, 2014 |
| Last update date |
Mar 12, 2015 |
| Contact name |
Ian Sudbery |
| E-mail(s) |
i.sudbery@sheffield.ac.uk
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| Organization name |
University of Sheffield
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| Department |
Molecular Biology and Biotechnology
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| Lab |
Sudbery Lab for Computational Genomics
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| Street address |
Firth Court, Western Bank
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| City |
Sheffield |
| ZIP/Postal code |
S11 8HG |
| Country |
United Kingdom |
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| Platforms (1) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA270862 |
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