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| Status |
Public on Jan 01, 2018 |
| Title |
Transcriptome analysis using customized AZ microarray on tomato Flower Abscission zone of transgeneic line TAPG::antisense TPRPgene |
| Organism |
Solanum lycopersicum |
| Experiment type |
Expression profiling by array
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| Summary |
To extend our previous knowledge from our gene expression studies on Flower pedicel Abscission zone using Affymetrix microarray chip we have employed whole transcriptome analysis by NGS as a discovery platform to identify and add the transcripts to pre-existing database. We designed the Customized AZ Microarray chip with the transcripts obtained from NGS (includes novel transcripts), pre existing Agilent probes, and some additional transcripts from previous databases. we developed transgenic lines by antisense silenciencing the genes, which are expressed in the AZ to study the functional role in abscission process. In the current stuty we silenced TPRP gene by antisense technology under abscisson promoter (TAPG::antsisense TPRP). The flower pedicel AZ tissue tissue was sampled at six time points (0, 4, 8, 12, 16 and 20 h), following flower removal, and analyzed for their gene expression profiles using the customized AZ microarray.
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| Overall design |
The flower pedicel AZ tissue tissue was sampled at six time points (0, 4, 8, 12, 16 and 20 h), following flower removal, and analyzed for their gene expression profiles using the customized AZ microarray.
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| Contributor(s) |
Sundaresan S, Meir S, Philosoph-Hadas S |
| Citation(s) |
29872533 |
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| Submission date |
Dec 31, 2014 |
| Last update date |
May 17, 2019 |
| Contact name |
Srivignesh Sundaresan |
| E-mail(s) |
srivignesh@volcani.agri.gov.il
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| Phone |
+972-3-9683367
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| Organization name |
Agricultural Research Organisation, The volcani center, Israel
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| Department |
Post Harvest Technology
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| Lab |
Dr. Shimon Meir Lab
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| Street address |
P.O.Box 6, Bet- Dagan
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| City |
Bet-Dagan |
| State/province |
Israel |
| ZIP/Postal code |
50250 |
| Country |
Israel |
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| Platforms (1) |
| GPL16798 |
Solanum lycopersicum Custom 4x180K Array designed by Genotypic Technology Private Limited. (AMADID:043310) |
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| Samples (12)
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| GSM1575355 |
TAPG:: antisense TPRP_Flower pedicel_ AZ_0hr_rep 1 |
| GSM1575356 |
TAPG:: antisense TPRP_Flower pedicel_ AZ_0hr_rep 2 |
| GSM1575357 |
TAPG:: antisense TPRP_Flower pedicel_ AZ_4hr_rep 1 |
| GSM1575358 |
TAPG:: antisense TPRP_Flower pedicel_ AZ_4hr_rep 2 |
| GSM1575359 |
TAPG:: antisense TPRP_Flower pedicel_ AZ_8hr_rep 1 |
| GSM1575360 |
TAPG:: antisense TPRP_Flower pedicel_ AZ_8hr_rep 2 |
| GSM1575361 |
TAPG:: antisense TPRP_Flower pedicel_ AZ_12hr_rep 1 |
| GSM1575362 |
TAPG:: antisense TPRP_Flower pedicel_ AZ_12hr_rep 2 |
| GSM1575363 |
TAPG:: antisense TPRP_Flower pedicel_ AZ_16hr_rep 1 |
| GSM1575364 |
TAPG:: antisense TPRP_Flower pedicel_ AZ_16hr_rep 2 |
| GSM1575365 |
TAPG:: antisense TPRP_Flower pedicel_ AZ_20hr_rep 1 |
| GSM1575366 |
TAPG:: antisense TPRP_Flower pedicel_ AZ_20hr_rep 2 |
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| Relations |
| BioProject |
PRJNA271401 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE64606_RAW.tar |
354.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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