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Series GSE64610 Query DataSets for GSE64610
Status Public on Jan 02, 2015
Title Differential gene transcript expression profiles among human monocyte-derived dendritic cells infected with Leishmania major Friedlin V1 strain and Leishmania donovani 1S strain parasites at early infection time points
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Monocyte derived dendritic cells (MDDC) were infected with Leishmania major or Leishmania donovani parasites and collected at 4, 8, and 24 hours post-infection to analyze the differential effects those parasite species have on human host cell gene expression over time.
 
Overall design Monocyte derived dendritic cells (MDDC) were generated from blood buffy coats collected from five anonymous healthy human donors and infected 10:1 (parasite to host cell) with Leishmania major Friedlin V1 strain or Leishmania donovani 1S strain parasites, where after 4, 8, or 24 hours total RNA was harvested from cells, cDNA generated, and hybridized to human gene transcipt expression arrays to assess differential host cell gene transcriptional expression differences relative to uninfected cells.
 
Contributor(s) Geraci NS, Harker B, Stephens M, Zeng E, McDowell MA
Citation(s) 25376316
Submission date Dec 31, 2014
Last update date May 20, 2016
Contact name Mary Ann McDowell
E-mail(s) mcdowell.11@nd.edu
Organization name University of Notre Dame
Department Biological Sciences
Street address 210 Galvin Life Science Center
City Notre Dame
State/province Indiana
ZIP/Postal code 46556
Country USA
 
Platforms (1)
GPL16025 NimbleGen Homo sapiens Expression Array [100718_HG18_opt_expr]
Samples (35)
GSM1575432 MDDC_Leishmania-donovani-strain-1S-infected_4HR-post-infection_donor-706
GSM1575433 MDDC_Leishmania-donovani-strain-1S-infected_8HR-post-infection_donor-706
GSM1575434 MDDC_Leishmania-donovani-strain-1S-infected_24HR-post-infection_donor-706
Relations
BioProject PRJNA271476

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64610_RAW.tar 112.2 Mb (http)(custom) TAR (of PAIR)
Processed data included within Sample table
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