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Status |
Public on Jul 31, 2015 |
Title |
Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage [RNA-seq] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
MyoD and NeuroD2 are master regulators of myogenesis and neurogenesis and bind to a "shared" E-box sequence (CAGCTG) and a "private" sequence (CAGGTG or CAGATG, respectively). To determine whether private-site recognition is sufficient to confer lineage-specification, we generated a MyoD-mutant with the DNA binding specificity of NeuroD2. Our results demonstrate that redirecting MyoD binding from MyoD-private sites to NeuroD2-private sites, despite preserved binding to the MyoD/NeuroD2-shared sites, is sufficient to change MyoD from a master regulator of myogenesis to a master regulator of neurogenesis.
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Overall design |
RNA-seq profiling of mouse P19 cells transfected with MyoD, NeuroD2 and chimera mutants. The chimeric mutants are MyoD with the bHLH domain replaced with the NeuroD2 bHLH domain.
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Contributor(s) |
Fong A, Yao Z, Zhong JW, Tapscott S |
Citation(s) |
25801030 |
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Submission date |
Jan 02, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Stephen Tapscott |
E-mail(s) |
stapscot@fredhutch.org
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Organization name |
Fred Hutch Cancer Research Center
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Department |
Human Biology
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Lab |
Tapscott
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Street address |
1100 Fairview N. Ave
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City |
Seattle |
State/province |
WASHINGTON |
ZIP/Postal code |
98103 |
Country |
USA |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (8)
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This SubSeries is part of SuperSeries: |
GSE64627 |
Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage |
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Relations |
BioProject |
PRJNA272314 |
SRA |
SRP051986 |