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Series GSE64670 Query DataSets for GSE64670
Status Public on Jan 06, 2015
Title EMT Alters Interstitial Fluid Flow-induced Signaling in ERBB2-positive Breast Cancer
Organism Homo sapiens
Experiment type Expression profiling by array
Summary A variety of mechanotransduction forces are altered in the tumor microenvironment (TME) and these biophysical forces can influence cancer progression. One such force is interstitial fluid flow (IFF) - the movement of fluid through the tissue matrix. IFF was previously shown to induce invasion of cancer cells, but the activated signaling cascades remain poorly understood. Here, it is demonstrated that IFF induces invasion of ERBB2/HER2 expressing breast cancer cells via activation of phosphoinositide-3-kinase (PI3K). In constitutively activate ERBB2 expressing cells that have undergone epithelial-to-mesenchymal transition (EMT), IFF-mediated invasion requires the chemokine receptor CXCR4, a gradient of its ligand CXCL12, and activity of the PI3K catalytic subunits p110a and β. In wild-type ERBB2 expressing cells, IFF-mediated invasion is chemokine receptor-independent and requires only p110a activation. To test whether cells undergoing EMT alter their signaling response to IFF, TGFb1 was used to induce EMT in wild-type ERBB2-expressing cells resulting in IFF-induced invasion dependent on CXCR4 and p110β.
Overall design 2 cell lines (NeuN, NeuT), 2 conditions (flow, static), 3 replicates each, 12 samples total
Contributor(s) Tchafa AM
Citation(s) 25566992
Submission date Jan 05, 2015
Last update date Aug 13, 2018
Contact name Alimatou M Tchafa
Phone 2672428234
Organization name UT Southwestern Medical Center
Department Biochemistry
Lab Hamid Mirzaei
Street address 5323 Harry Hines Blvd, Rm Y4.306
City Dallas
State/province TEXAS
ZIP/Postal code 75390
Country USA
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (12)
GSM1576692 NT_S1
GSM1576693 NT_S2
GSM1576694 NT_S3
BioProject PRJNA271605

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64670_Non-normalized_data.txt.gz 3.6 Mb (ftp)(http) TXT
GSE64670_RAW.tar 26.2 Mb (http)(custom) TAR
Processed data included within Sample table

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