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Status |
Public on Jan 07, 2015 |
Title |
Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols |
Organisms |
Drosophila melanogaster; Drosophila virilis |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We sequenced mRNA according to several library prep protocols with known mixtures of two species of Drosophila in order to establish linear response in each protocol.
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Overall design |
For each library prep protocol, mixtures with 0%, 5%, 10%, and 20% D. virilis total RNA was prepared, then libraries prepared according to instructions.
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Contributor(s) |
Combs PA, Eisen MB |
Citation(s) |
25834775 |
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Submission date |
Jan 05, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Peter Acuña Combs |
E-mail(s) |
pcombs@stanford.edu
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Organization name |
Stanford University
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Department |
Biology
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Lab |
Hunter Fraser
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Street address |
Gilbert Rm 309, 371 Serra Mall
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City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
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Platforms (1) |
GPL19602 |
Illumina HiSeq 2000 (Drosophila melanogaster; Drosophila virilis) |
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Samples (26)
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Relations |
BioProject |
PRJNA271674 |
SRA |
SRP051726 |