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| Status |
Public on Aug 01, 2015 |
| Title |
Influence of low and high force muscle activity on paralyzed muscle gene expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We used a novel approach to study the acute effect of two frequencies of stimulation (20 Hz and 5 Hz; high and low force, respectively) on gene regulation in people with chronic paralysis. Three hours after the completion of the electrical stimulation protocol (5 Hz or 20 Hz), we sampled the vastus lateralis muscle and examined genes involved with metabolic transcription, glycolysis, oxidative phosphorylation, and mitochondria remodelling. We discovered that the 5 Hz stimulation, despite generating a lower overall force, induced a 5-6 fold increase (p<0.05) in key metabolic transcription factors including PGC-1α, NR4A3, and ABRA. Neither protocol showed a robust regulation of genes for glycolysis, oxidative phosphorylation, and mitochondria remodelling.
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| Overall design |
We analyzed skeletal muscle using the Affymetrix Human Exon 1.0 ST platform. Array data was processed by Partek Genomic Suites.
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| Contributor(s) |
Petrie MA, Suneja M, Shields RK |
| Citation(s) |
25635001 |
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| Submission date |
Jan 05, 2015 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Richard Shields |
| Organization name |
The University of Iowa
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| Street address |
1-252 Medical Education Building
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| City |
Iowa City |
| ZIP/Postal code |
52242 |
| Country |
USA |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (24)
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| GSM1576824 |
Skeletal Muscle Sample 1 (experiment, high-force) |
| GSM1576825 |
Skeletal Muscle Sample 2 (control, low-force) |
| GSM1576826 |
Skeletal Muscle Sample 3 (control, high-force) |
| GSM1576827 |
Skeletal Muscle Sample 4 (experiment, low-force) |
| GSM1576828 |
Skeletal Muscle Sample 5 (experiment, high-force) |
| GSM1576829 |
Skeletal Muscle Sample 6 (control, low-force) |
| GSM1576830 |
Skeletal Muscle Sample 7 (control, high-force) |
| GSM1576831 |
Skeletal Muscle Sample 8 (experiment, low-force) |
| GSM1576832 |
Skeletal Muscle Sample 9 (experiment, high-force) |
| GSM1576833 |
Skeletal Muscle Sample 10 (control, low-force) |
| GSM1576834 |
Skeletal Muscle Sample 11 (control, high-force) |
| GSM1576835 |
Skeletal Muscle Sample 12 (experiment, low-force) |
| GSM1576836 |
Skeletal Muscle Sample 13 (experiment, high-force) |
| GSM1576837 |
Skeletal Muscle Sample 14 (control, low-force) |
| GSM1576838 |
Skeletal Muscle Sample 15 (control, high-force) |
| GSM1576839 |
Skeletal Muscle Sample 16 (experiment, low-force) |
| GSM1576840 |
Skeletal Muscle Sample 17 (experiment, high-force) |
| GSM1576841 |
Skeletal Muscle Sample 18 (control, low-force) |
| GSM1576842 |
Skeletal Muscle Sample 19 (control, high-force) |
| GSM1576843 |
Skeletal Muscle Sample 20 (experiment, low-force) |
| GSM1576844 |
Skeletal Muscle Sample 21 (experiment, high-force) |
| GSM1576845 |
Skeletal Muscle Sample 22 (control, low-force) |
| GSM1576846 |
Skeletal Muscle Sample 23 (control, high-force) |
| GSM1576847 |
Skeletal Muscle Sample 24 (experiment, low-force) |
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| Relations |
| BioProject |
PRJNA271619 |
| Supplementary data files not provided |
| Processed data included within Sample table |
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