|
| Status |
Public on Feb 22, 2016 |
| Title |
Elucidating the Linezolid response of Staphylococcus aureus USA300 by a holistic study |
| Platform organisms |
Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus USA300 |
| Sample organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by array
|
| Summary |
The translation inhibitor Linezolid is an important antibiotic of last resort against multiresistant gram-positive pathogens including MRSA. Linezolid is reported to specifically inhibit extracellular virulence factors, but the molecular cause is unknown. To elucidate the physiological response of S. aureus to Linezolid in general and the possible inhibition of virulence factors specifically we performed a holistic study. We added Linezolid to logarithmically growing S. aureus cells and analyzed the Linezolid stress response with transcriptomics, quantitative proteomics and microscopy experiments.
As previously observed in studies on other translation inhibitors S. aureus is adapting its protein biosynthesis machinery to the reduced translation efficiency, for example the synthesis of ribosomal proteins is induced. But we also observed unexpected results like a general decline in the amount of extracellular and membrane proteins. In addition cell shape and size changed after Linezolid stress and cell division was diminished. Finally, the chromosome condensed after LZD stress and lost contact to the membrane.
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| |
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| Overall design |
sample versus pool design (pool = mixture of equal amounts of all RNA samples analyzed), all RNA samples were isolated as biological triplicates
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| |
|
| Contributor(s) |
Bonn F, Schaffer M, Mäder U, Völker U, Becher D |
| Citation(s) |
26996810 |
| |
| Submission date |
Jan 22, 2015 |
| Last update date |
Feb 27, 2019 |
| Contact name |
Florian Bonn |
| E-mail(s) |
florian.bonn@uni-greifswald.de
|
| Organization name |
University of Greifswald
|
| Department |
Institute for Microbiology
|
| Lab |
Professor Becher
|
| Street address |
Jahnstraße 15
|
| City |
Greifswald |
| ZIP/Postal code |
17489 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL7137 |
Agilent-017903 Staphylococcus aureus V5 Bis 15K (basic) |
|
| Samples (15)
|
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| Relations |
| BioProject |
PRJNA273405 |
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