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| Status |
Public on Jan 01, 2016 |
| Title |
Expression profiling of E14 cells overexpressing Hk2/Pkm2 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: In this study, we compared transcriptome profiling (RNA-seq) between normal mouse embryonic stem cell (E14) and Hexokinase2 (Hk2)/ Pyruvate Kinase M2 (Pkm2) overexpressed E14 cell.
Result: Using an optimized data analysis workflow, we mapped over 4 billion sequence reads per sample to the mouse genome (build mm9) and identified 28698 transcripts in 5 samples.
Conclusion: Our study represents the first detailed analysis of Hk2/ Pkm2 overexpressed E14 cell transcriptomes, generated by RNA-seq technology
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| Overall design |
We compared transcriptome profiling (RNA-Seq) between normal mouse embryonic stem cell (E14) and E14 cells over-expressing Hexokinase2 (Hk2)/Pyruvate Kinase M2 (Pkm2)
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| |
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| Contributor(s) |
Kim H, Youn H |
| Citation(s) |
26059508 |
| |
| Submission date |
Jan 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Hyunsoo Kim |
| E-mail(s) |
khsoo82@snu.ac.kr
|
| Organization name |
Seoul National University
|
| Street address |
103 Daehakro, Jongro-gu
|
| City |
Seoul |
| ZIP/Postal code |
110-799 |
| Country |
South Korea |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA273493 |
| SRA |
SRP052789 |
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