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| Status |
Public on Jan 19, 2019 |
| Title |
Tristetraprolin (TTP) regulated splenic transcriptome in "TTPΔARE" mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Tristetraprolin (TTP) binds to specific AU-rich elements in the 3'UTR of certain transcripts and regulates post-transcriptional gene expression by increasing the rate of mRNA turnover. Here, we generated a novel TTP mouse line (TTPΔARE) with a 136 base deletion in the 3'UTR AU-rich region of TTP mRNA, which resulted in a moderate increase in TTP protein expression in many tissues. In this study, we evaluated the effects of the moderate TTP overexpression on the overall pattern of gene expression in spleen, to investigate the effect of this mutation on mRNA targets of TTP under normal conditions. We utilized "TTPΔARE" (136 b deletion in the 3'UTR AU-rich region of Zfp36 mRNA) mice that overexpress TTP and compared the transcriptomic changes to the littermate "WT" mice. Spleen mRNA from four WT and four "TTPΔARE" mice were subjected to mRNA-Seq. All the animals used in this study were males between the ages of 8-12 weeks and were on a C57BL/6 background.
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| Overall design |
Examination of gene expression differences in mouse spleen from 4 wild-type and 4 TTPΔARE male mice aged 8-12 weeks
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| Contributor(s) |
Blackshear PJ, Patial S, Huang W, Stumpo D, Flake G |
| Citation missing |
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| |
| Submission date |
Jan 27, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Perry J Blackshear |
| E-mail(s) |
black009@niehs.nih.gov
|
| Phone |
919-541-4926
|
| Organization name |
NIEHS
|
| Street address |
101 Alexander Dr.
|
| City |
Durham |
| ZIP/Postal code |
27709 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA273758 |
| SRA |
SRP052881 |
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