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| Status |
Public on Jan 31, 2015 |
| Title |
A Nested Parallel Experiment Demonstrates Differences in Intensity-Dependence Between RNA-Seq and Microarrays |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
We carried out a parallel nested experiment performed simultaneously on RNA-Seq and microarrays that systematically split variation into four stages (treatment, biological variation, library preparation, and chip/lane noise), allowing a separation and comparison of the sources of variation in a well-controlled cellular system, Saccharomyces cerevisiae.
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| Overall design |
Nested design. Two conditions (E for ethanol and G for glucose as sole carbon source), each grown on two biological replicates (1 and 2), prepared in two technical preparations (A and B), then hybridized to two chips (1 and 2)
This submission represents microarray component of study.
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| Contributor(s) |
Robinson DG, Wang JY |
| Citation(s) |
26130709 |
| BioProject |
PRJNA271248 |
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| Submission date |
Jan 28, 2015 |
| Last update date |
Jul 06, 2015 |
| Contact name |
David Garrett Robinson |
| E-mail(s) |
dgrtwo@princeton.edu
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| Organization name |
Princeton University
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| Department |
Lewis-Sigler Institute
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| Lab |
Storey Lab
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| Street address |
Washington Road
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| City |
Princeton |
| State/province |
New Jersey |
| ZIP/Postal code |
08544 |
| Country |
USA |
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| Platforms (1) |
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| Samples (16)
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| GSM1593819 |
E1A1 - Condition: ethanol, Biological Replicate: 1, Preparation: A, Chip 1 |
| GSM1593820 |
E1A2 - Condition: ethanol, Biological Replicate: 1, Preparation: A, Chip 2 |
| GSM1593821 |
E1B1 - Condition: ethanol, Biological Replicate: 1, Preparation: B, Chip 1 |
| GSM1593822 |
E1B2 - Condition: ethanol, Biological Replicate: 1, Preparation: B, Chip 2 |
| GSM1593823 |
E2A1 - Condition: ethanol, Biological Replicate: 2, Preparation: A, Chip 1 |
| GSM1593824 |
E2A2 - Condition: ethanol, Biological Replicate: 2, Preparation: A, Chip 2 |
| GSM1593825 |
E2B1 - Condition: ethanol, Biological Replicate: 2, Preparation: B, Chip 1 |
| GSM1593826 |
E2B2 - Condition: ethanol, Biological Replicate: 2, Preparation: B, Chip 2 |
| GSM1593827 |
G1A1 - Condition: glucose, Biological Replicate: 1, Preparation: A, Chip 1 |
| GSM1593828 |
G1A2 - Condition: glucose, Biological Replicate: 1, Preparation: A, Chip 2 |
| GSM1593829 |
G1B1 - Condition: glucose, Biological Replicate: 1, Preparation: B, Chip 1 |
| GSM1593830 |
G1B2 - Condition: glucose, Biological Replicate: 1, Preparation: B, Chip 2 |
| GSM1593831 |
G2A1 - Condition: glucose, Biological Replicate: 2, Preparation: A, Chip 1 |
| GSM1593832 |
G2A2 - Condition: glucose, Biological Replicate: 2, Preparation: A, Chip 2 |
| GSM1593833 |
G2B1 - Condition: glucose, Biological Replicate: 2, Preparation: B, Chip 1 |
| GSM1593834 |
G2B2 - Condition: glucose, Biological Replicate: 2, Preparation: B, Chip 2 |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE65365_RAW.tar |
97.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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