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| Status |
Public on Jan 29, 2018 |
| Title |
Expression profiling of human valve interstitial and endothelial cells and the effect of BMP and TGFB signaling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Microarray gene expression profiling of the minimally cultured human heart valve interstitial and endothelial cells with and without treatment with BMP4 and TGFB3 signaling molecules
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| Overall design |
We collected heart valve tissues from explanted non-diseased heart valve from pediatric patients undergoing heart transplant. Valve interstitial cells (VICs) and valve endothelial cells (VECs) were harvested and expanded in a culturing system for a few passages. These cells either treated with BMP4 or TGFb3 signaling agonists or untreated (control) and incubated for 8 hours before used for microarray profiling experiments. The goal is to identify (1) the gene expression signatures associated with human heart valve interstitial and endothelial cells, and (2) the effect of BMP and TGFb signaling signaling on these cells, especially with regard to promoting/inhibiting the endothelial-mesenchymal transformation (EMT) program.
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| Contributor(s) |
Bayoumi AS, Ho JW |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 30, 2015 |
| Last update date |
Jan 29, 2018 |
| Contact name |
Joshua Ho |
| E-mail(s) |
j.ho@victorchang.edu.au
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| Phone |
+61292958645
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| Organization name |
Victor Chang Cardiac Research Institute
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| Lab |
Bioinformatics and Systems Medicine Laboratory
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| Street address |
Lowy Packer Building
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| City |
Darlinghurst |
| State/province |
NSW |
| ZIP/Postal code |
2010 |
| Country |
Australia |
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| Platforms (1) |
| GPL10904 |
Illumina HumanHT-12 V4.0 expression beadchip (gene symbol) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA274026 |
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