|
| Status |
Public on May 21, 2015 |
| Title |
Purification and transcriptomic analysis of mouse fetal Leydig cells reveals candidate genes for disorders of sex development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To examine the transcriptome of early testicular somatic cells during gonadogenesis at 12.5dpc RNA sequencing (RNA-Seq) was performed on murine primary testicular cell lineages isolated from the Sf1-eGFP line by FACS. The three main somatic cell lineages of the testis were isolated: the Sertoli cells which direct male development; the fetal Leydig cells (FLCs) that produce steroid hormones and virilise the XY individual and a heterogenous population of interstitial cells, some of which give rise to the adult Leydig cells (ALCs). This dataset provides a platform for exploring the biology of FLCs and understanding the role of these cells in testicular development and masculinization of the embryo, and a basis for targeted studies designed to identify causes of idiopathic XY DSD.
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| |
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| Overall design |
RNA-Seq of 3 enriched cell populations from 12.5dpc mouse gonad (Sertoli cells, Leydig cells and Interstitial cells isolated by FACS-sorting) on an Illumina HiSeq 1500, in triplicate.
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| Contributor(s) |
McClelland K, Larney C, Koopman P, Bowles J, Bell K, Oshlack A |
| Citation(s) |
25855264 |
| |
| Submission date |
Feb 02, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Katrina M Bell |
| E-mail(s) |
katrina.bell@mcri.edu.au
|
| Phone |
61399366709
|
| Organization name |
MCRI
|
| Street address |
RCH, Flemington Rd, Parkville
|
| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA274224 |
| SRA |
SRP053038 |
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