|
| Status |
Public on Mar 01, 2015 |
| Title |
Lin28b promotes fetal B lymphopoiesis through the transcription factor Arid3a [miRNA] |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Mouse B cell precursors from fetal liver and adult bone marrow generate distinctive B cell progeny when transplanted into immunodeficient recipients, supporting a two-pathway model for B lymphopoiesis, fetal “B-1” and adult “B-2”. Recently Lin28b was shown to be important for the switch between fetal and adult pathways; however, neither the mechanism of Lin28b action nor the importance of BCR signaling in this process was addressed. Here we report important advances in our understanding of the regulation of B 1/B-2 development.
First, modulation of Let-7 in fetal Pro-B cells is sufficient to alter fetal B-1 development to produce B cells resembling the progeny of adult B-2 development. Second, intact BCR signaling is required for generation of B1a B cells from Lin28b-transduced bone marrow progenitors, supporting a requirement for ligand-dependent selection, as is the case for normal B1a B cells. Third, the VH repertore of Lin28b-induced bone marrow B1a B cells differs from that of normal B1a. Finally we identify the Arid3a transcription factor as a key target of Let-7, whose ectopic expression is sufficient to induce B-1 development in adult Pro-B cells and whose knockdown blocks B-1 development in fetal Pro-B cells.
|
| |
|
| Overall design |
3 individual sorts of Fr.B/C fractions from bone marrow and fetal liver of BALB/c mice
|
| |
|
| Contributor(s) |
Zhou Y, Li Y, Bandi S, Tang L, Shinton SA, Hayakawa K, Hardy RR |
| Citation(s) |
25753579 |
| |
| Submission date |
Feb 02, 2015 |
| Last update date |
May 21, 2015 |
| Contact name |
Kyoko Hayakawa |
| E-mail(s) |
kyoko.hayakawa@fccc.edu
|
| Organization name |
Fox Chase Cancer Center
|
| Street address |
333 Cottman Avenue
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19111 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL7732 |
Agilent-019119 Mouse miRNA Microarray 1.0 G4472A |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE65536 |
Lin28b promotes fetal B lymphopoiesis through the transcription factor Arid3a |
|
| Relations |
| BioProject |
PRJNA274291 |
Less...
More...