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Status |
Public on Mar 01, 2015 |
Title |
Lin28b promotes fetal B lymphopoiesis through the transcription factor Arid3a [miRNA] |
Organism |
Mus musculus |
Experiment type |
Non-coding RNA profiling by array
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Summary |
Mouse B cell precursors from fetal liver and adult bone marrow generate distinctive B cell progeny when transplanted into immunodeficient recipients, supporting a two-pathway model for B lymphopoiesis, fetal “B-1” and adult “B-2”. Recently Lin28b was shown to be important for the switch between fetal and adult pathways; however, neither the mechanism of Lin28b action nor the importance of BCR signaling in this process was addressed. Here we report important advances in our understanding of the regulation of B 1/B-2 development. First, modulation of Let-7 in fetal Pro-B cells is sufficient to alter fetal B-1 development to produce B cells resembling the progeny of adult B-2 development. Second, intact BCR signaling is required for generation of B1a B cells from Lin28b-transduced bone marrow progenitors, supporting a requirement for ligand-dependent selection, as is the case for normal B1a B cells. Third, the VH repertore of Lin28b-induced bone marrow B1a B cells differs from that of normal B1a. Finally we identify the Arid3a transcription factor as a key target of Let-7, whose ectopic expression is sufficient to induce B-1 development in adult Pro-B cells and whose knockdown blocks B-1 development in fetal Pro-B cells.
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Overall design |
3 individual sorts of Fr.B/C fractions from bone marrow and fetal liver of BALB/c mice
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Contributor(s) |
Zhou Y, Li Y, Bandi S, Tang L, Shinton SA, Hayakawa K, Hardy RR |
Citation(s) |
25753579 |
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Submission date |
Feb 02, 2015 |
Last update date |
May 21, 2015 |
Contact name |
Kyoko Hayakawa |
E-mail(s) |
kyoko.hayakawa@fccc.edu
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Organization name |
Fox Chase Cancer Center
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Street address |
333 Cottman Avenue
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City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19111 |
Country |
USA |
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Platforms (1) |
GPL7732 |
Agilent-019119 Mouse miRNA Microarray 1.0 G4472A |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE65536 |
Lin28b promotes fetal B lymphopoiesis through the transcription factor Arid3a |
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Relations |
BioProject |
PRJNA274291 |