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| Status |
Public on Mar 28, 2015 |
| Title |
Genome-wide analysis of mRNA expression in brat mutant versus w1118 early Drosophila embryos |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
mRNA expression was assessed genome-wide in brat mutant and w1118 early Drosophila embryos, to elucidate the role of BRAT in regulating mRNA stability. Four different time-points were analyzed for each genotype: 0-to-1.5 hours post-egglaying, 1.5-to-3.0 hours post-egglaying, 3.0-to-4.5 hours post-egglaying, and 4.5-to-6.0 hours post-egglaying.
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| Overall design |
There are a total of 24 samples. This includes 3 biological replicates for each of the following: 1) 0-to-1.5 hour brat mutant embryos, 2) 1.5-to-3.0 hour brat mutant embryos, 3) 3.0-to-4.5 hour brat mutant embryos, 4) 4.5-to-6.0 hour brat mutant embryos, 5) 0-to-1.5 hour w1118 embryos, 6) 1.5-to-3.0 hour w1118 embryos, 7) 3.0-to-4.5 hour w1118 embryos, and 8) 4.5-to-6.0 hour w1118 embryos.
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| Contributor(s) |
Laver JD, Li X, Ray D, Cook KB, Hahn NA, Nabeel-Shah S, Kekis M, Luo H, Marsolais AJ, Fung K, Hughes TR, Westwood JT, Sidhu SS, Morris Q, Lipshitz HD, Smibert CA |
| Citation(s) |
25962635 |
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| Submission date |
Feb 05, 2015 |
| Last update date |
Jul 06, 2015 |
| Contact name |
Howard D Lipshitz |
| E-mail(s) |
howard.lipshitz@utoronto.ca
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| Organization name |
University of Toronto
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| Department |
Molecular Genetics
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| Street address |
1 King's College Circle
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| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5S1A8 |
| Country |
Canada |
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| Platforms (1) |
| GPL19743 |
NimbleGen Drosophila melanogaster 145K 12plex custom expression array (080523_D_melanogaster_5.5_expr gene version) |
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| Samples (24)
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| GSM1602628 |
Drosophila_melanogaster_brat_mutant_0-1.5h_embryos_rep1 |
| GSM1602629 |
Drosophila_melanogaster_brat_mutant_0-1.5h_embryos_rep2 |
| GSM1602630 |
Drosophila_melanogaster_brat_mutant_0-1.5h_embryos_rep3 |
| GSM1602631 |
Drosophila_melanogaster_brat_mutant_1.5-3h_embryos_rep1 |
| GSM1602632 |
Drosophila_melanogaster_brat_mutant_1.5-3h_embryos_rep2 |
| GSM1602633 |
Drosophila_melanogaster_brat_mutant_1.5-3h_embryos_rep3 |
| GSM1602634 |
Drosophila_melanogaster_brat_mutant_3-4.5h_embryos_rep1 |
| GSM1602635 |
Drosophila_melanogaster_brat_mutant_3-4.5h_embryos_rep2 |
| GSM1602636 |
Drosophila_melanogaster_brat_mutant_3-4.5h_embryos_rep3 |
| GSM1602637 |
Drosophila_melanogaster_brat_mutant_4.5-6h_embryos_rep1 |
| GSM1602638 |
Drosophila_melanogaster_brat_mutant_4.5-6h_embryos_rep2 |
| GSM1602639 |
Drosophila_melanogaster_brat_mutant_4.5-6h_embryos_rep3 |
| GSM1602640 |
Drosophila_melanogaster_w1118_0-1.5h_embryos_rep1 |
| GSM1602641 |
Drosophila_melanogaster_w1118_0-1.5h_embryos_rep2 |
| GSM1602642 |
Drosophila_melanogaster_w1118_0-1.5h_embryos_rep3 |
| GSM1602643 |
Drosophila_melanogaster_w1118_1.5-3h_embryos_rep1 |
| GSM1602644 |
Drosophila_melanogaster_w1118_1.5-3h_embryos_rep2 |
| GSM1602645 |
Drosophila_melanogaster_w1118_1.5-3h_embryos_rep3 |
| GSM1602646 |
Drosophila_melanogaster_w1118_3-4.5h_embryos_rep1 |
| GSM1602647 |
Drosophila_melanogaster_w1118_3-4.5h_embryos_rep2 |
| GSM1602648 |
Drosophila_melanogaster_w1118_3-4.5h_embryos_rep3 |
| GSM1602649 |
Drosophila_melanogaster_w1118_4.5-6h_embryos_rep1 |
| GSM1602650 |
Drosophila_melanogaster_w1118_4.5-6h_embryos_rep2 |
| GSM1602651 |
Drosophila_melanogaster_w1118_4.5-6h_embryos_rep3 |
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| Relations |
| BioProject |
PRJNA274641 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE65661_RAW.tar |
54.6 Mb |
(http)(custom) |
TAR (of PAIR) |
| Processed data included within Sample table |
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