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| Status |
Public on Jan 01, 2017 |
| Title |
Glucocorticoid-regulated circadian expression genes in spinal cord of nerve-injured mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We prepared adrenalectomized (ADX) and sham-operated (sham) mice kept under a light-controlled room (ZT, zeitgeber time; ZT0, light on; ZT12, light off), and thereafter their sciatic nerve was partially ligated; mice undergone partial sciatic nerve ligation (PSL) was used as an animal model of neuropathic pain. To screen pain-related genes whose spinal expression exhibits circadian oscillation in a glucocorticoid-dependent manner, we performed oligonucleotide microarray analyses using RNA isolated from the spinal cords of sham-PSL mice or ADX-PSL mice at ZT10 and ZT22 when circulating glucocorticoid levels in sham-PSL mice peaked and declined, respectively
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| Overall design |
Samples were corrected from spinal cords of sham-PSL mice or ADX-PSL mice at ZT10 and ZT22 when circulating glucocorticoid levels in sham-PSL mice peaked and declined, respectively
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| Contributor(s) |
Koyanagi S, Kusunose N, Matsunaga N, Ohdo S |
| Citation(s) |
27739425 |
| |
| Submission date |
Feb 09, 2015 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Satoru Koyanagi |
| E-mail(s) |
skoyanagi@icloud.com
|
| Organization name |
Kyushu University
|
| Department |
Pharmaceutics
|
| Street address |
3-1-1 Maidashi, Higashi-ku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA274958 |
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